Optimization of the Microwave-Assisted Extraction Process for Polysaccharides in Himematsutake (Agaricus blazei Murrill) and Evaluation of Their Antioxidant Activities

被引:17
作者
Zhang, Zuofa [1 ]
Lv, Guoying [1 ]
Pan, Huijuan [1 ]
Shi, Liangen [2 ]
Fan, Leifa [1 ]
机构
[1] Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Anim Sci, Hangzhou 310021, Zhejiang, Peoples R China
关键词
microwave-assisted extraction; polysaccharides; Agaricus blazei Murrill; antioxidant activity; MOLECULAR-WEIGHT POLYSACCHARIDES; ULTRASONIC TREATMENT; DERIVATIVES;
D O I
10.3136/fstr.17.461
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Microwave-assisted extraction (MAE) technique was employed for the extraction of himematsutake (Agaricus blazei Murrill) polysaccharide (PMAE) and optimized by Box-Behnken design. The optimum conditions were extraction time 29.37 min, microwave power 400 W, extraction temperature 74.64 degrees C and ratio of water to material 32.7:1 with an enhanced yield of 12.35%. Furthermore, PMAE had increased antioxidant activity in various oxidative systems in vitro when compared to polysaccharides extracted by conventional methods. The data obtained clearly showed that MAE is a fine way to extract the polysaccharides in A. blazei Murrill. Further, PMAE may be developed into a functional food as well as potential therapeutic agent.
引用
收藏
页码:461 / 470
页数:10
相关论文
共 30 条
[1]  
BAUMANN J, 1979, Naunyn-Schmiedeberg's Archives of Pharmacology, V308, pR27
[2]   REACTIVE SPECIES AND THEIR ACCUMULATION ON RADICAL-DAMAGED PROTEINS [J].
DEAN, RT ;
GIESEG, S ;
DAVIES, MJ .
TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (11) :437-441
[3]  
Ding Q, 1998, CHINESE J POLYM SCI, V16, P62
[4]   ACTION OF PHENOLIC DERIVATIVES (ACETAMINOPHEN, SALICYLATE, AND 5-AMINOSALICYLATE) AS INHIBITORS OF MEMBRANE LIPID-PEROXIDATION AND AS PEROXYL RADICAL SCAVENGERS [J].
DINIS, TCP ;
MADEIRA, VMC ;
ALMEIDA, LM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 315 (01) :161-169
[5]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[6]  
Eskilsson CS, 2000, J CHROMATOGR A, V902, P227
[7]   Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum [J].
Fan, Yijun ;
He, XingJin ;
Zhou, Songdong ;
Luo, Aoxue ;
He, Tao ;
Chun, Ze .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2009, 45 (02) :169-173
[8]   Antioxidant activity of different phenolic fractions separated from an Italian red wine [J].
Ghiselli, A ;
Nardini, M ;
Baldi, A ;
Scaccini, C .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (02) :361-367
[9]  
Gordon MH, 1990, FOOD ANTIOXIDANTS, V1, P18, DOI DOI 10.1007/978-94-009-0753-9_1
[10]  
Halliwell B., 1991, FREE RADICALS FOOD A, P37