Identification and characterization of the Streptomyces globisporus 1912 regulatory gene IndYR that affects sporulation and antibiotic production

被引：20

作者：

Ostash, Bohdan ^{[1
]}

Rebets, Yuriy ^{[1
,2
,3
]}

Myronovskyy, Maksym ^{[1
]}

Tsypik, Olga ^{[1
]}

Ostash, Iryna ^{[1
]}

Kulachkovskyy, Oleksandr ^{[1
]}

Datsyuk, Yuriy ^{[4
]}

Nakamura, Tatsunosuke ^{[3
]}

Walker, Suzanne ^{[2
]}

Fedorenko, Victor ^{[1
]}

机构：

[1] Ivan Franko Natl Univ Lviv, Dept Genet & Biotechnol, UA-79005 Lvov, Ukraine

[2] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA

[3] Niigata Univ Pharm & Appl Life Sci, Dept Microbiol, Niigata 9568603, Japan

[4] Ivan Franko Natl Univ Lviv, Dept Phys Earth, UA-79005 Lvov, Ukraine

来源：

MICROBIOLOGY-SGM | 2011年 / 157卷

基金：

美国国家卫生研究院;

关键词：

LANDOMYCIN-E; GNTR-FAMILY; TRANSCRIPTIONAL REGULATOR; COELICOLOR A3(2); ABC TRANSPORTER; PROTEIN; DNA; BIOSYNTHESIS; EXPRESSION; RESISTANCE;

D O I：

10.1099/mic.0.045088-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here, we report the identification and functional characterization of the Streptomyces globisporus 1912 gene IndYR, which encodes a GntR-like regulator of the YtrA subfamily. Disruption of IndYR arrested sporulation and antibiotic production in S. globisporus. The results of in vivo and in vitro studies revealed that the ABC transporter genes IndW-IndW2 are targets of LndYR repressive action. In Streptomyces coelicolor M145, IndYR overexpression caused a significant increase in the amount of extracellular actinorhodin. We suggest that IndYR controls the transcription of transport system genes in response to an as-yet-unidentified signal. Features that distinguish IndYR-based regulation from other known regulators are discussed.

引用

页码：1240 / 1249

页数：10

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