Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking

被引：50

作者：

Kim, Ekyune ^{[1
]}

Lee, Youngjeon ^{[1
,2
]}

Lee, Hyun-Ju ^{[4
]}

Kim, Ji Su ^{[1
]}

Song, Bong-Seok ^{[1
]}

Huh, Jae-Won ^{[1
]}

Lee, Sang-Rae ^{[1
]}

Kim, Sun-Uk ^{[1
]}

Kim, Sang-Hyun ^{[1
]}

Hong, Yonggeun ^{[2
,3
]}

Shim, Insop ^{[4
]}

Chang, Kyu-Tae ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Natl Primate Res Ctr, Ochang Eup 363883, Chung Buk, South Korea

[2] Inje Univ, Dept Rehabil Sci, Interdisciplinary PhD Program, Coll Biomed Sci & Engn, Gyeongnam, South Korea

[3] Inje Univ, Cardiovasc & Metab Dis Ctr, Coll Biomed Sci & Engn, Gyeongnam, South Korea

[4] Kyung Hee Univ, Acupuncture & Meridian Sci Res Ctr, Coll Oriental Med, Seoul, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 403卷 / 02期

关键词：

Retromer complex; Vacuolar protein sorting 26b (Vps26b); Vps26b-deficient mice; Endosomes; trans-Golgi network (TGN); MAMMALIAN RETROMER; RECEPTOR; GENE; IDENTIFICATION; COMPONENT; PROTEINS; SURFACE; ADAM1A; SPERM;

D O I：

10.1016/j.bbrc.2010.10.121

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The retromer complex, which mediates retrograde transport from endosomes to the trans-Golgi network, is a heteropentameric complex that contains a multifunctional cargo recognition heterotrimer consisted of the vacuolar protein sorting (Vps) subunits Vps26, Vps29, and Vps35. In mammals, there are two different isoforms of Vps26, Vps26a and Vps26b, that localize to the endosome, and to the plasma membrane, respectively. To elucidate the biological significance of the Vps26b isoform, we generated Vps26b knockout mice and studied their molecular, histological, and behavioral phenotypes. We found that the loss of Vps26b results in no significant defects in the behavior, body size, and health of the mice. Vps26b-deficient mice showed a severe reduction of Vps35 protein at cellular level and lacked the Vps26b-Vps29-Vps35 retromer complex, despite the normal presence of the Vps26a-Vps29-Vps35 retromer complex. Relatively, the amount of sortilin was increased approximately 20% in the Vps26b-deficient mice, whereas the sorLA was normal. These results suggest that mouse Vps26b-Vps29-Vps35 retromer complex is implicated in the transport of sortilin from endosomes to the trans-Golgi network (TGN). (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：167 / 171

页数：5