IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth

被引：19

作者：

Ayars, Michael ^{[1
]}

O'Sullivan, Eileen ^{[1
]}

Macgregor-Das, Anne ^{[1
]}

Shindo, Koji ^{[1
]}

Kim, Haeryoung ^{[1
]}

Borges, Michael ^{[1
]}

Yu, Jun ^{[1
]}

Hruban, Ralph H. ^{[1
,2
]}

Goggins, Michael ^{[1
,2
,3
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Sol Goldman Pancreat Canc Res Ctr, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Oncol, Sol Goldman Pancreat Canc Res Ctr, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Med, Sol Goldman Pancreat Canc Res Ctr, Baltimore, MD 21205 USA

来源：

ONCOTARGET | 2017年 / 8卷 / 48期

关键词：

pancreatic cancer; PanIN; RNA-seq; IL2RG; JAK3; PRECURSOR LESIONS; EXPRESSION; CHAIN; BETA; REGULATOR; TP53;

D O I：

10.18632/oncotarget.19848

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Pancreatic ductal adenocarcinoma evolves from precursor lesions, the most common of which is pancreatic intraepithelial neoplasia (PanIN). We performed RNA-sequencing analysis of laser capture microdissected PanINs and normal pancreatic duct cells to identify differentially expressed genes between PanINs and normal pancreatic duct, and between low-grade and high-grade PanINs. One of the most highly overexpressed transcripts identified in PanIN is interleukin-2 receptor subunit gamma (IL2RG) encoding the common gamma chain, IL2R gamma. CRISPR-mediated knockout of IL2RG in orthotopically implanted pancreatic cancer cells resulted in attenuated tumor growth in mice and reduced JAK3 expression in orthotopic tumors. These results indicate that IL2R gamma/JAK3 signaling contributes to pancreatic cancer cell growth in vivo.

引用

页码：83370 / 83383

页数：14

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