Oxygen Reduction Reaction Activity and Stability of Electrochemically Deposited Bilirubin Oxidase

被引:2
|
作者
Takimoto, Daisuke [1 ,2 ]
Tsujimura, Seiya [1 ]
机构
[1] Univ Tsukuba, Fac Pure & Appl Sci, Div Mat Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058573, Japan
[2] Shinshu Univ, Ctr Energy & Environm Sci, 3-15-1 Tokida, Ueda, Nagano 3868567, Japan
基金
日本学术振兴会;
关键词
Electrochemical deposition; Bilirubin oxidase; Stability; DIRECT ELECTRON-TRANSFER; TRANSFER-TYPE BIOELECTROCATALYSIS; CARBON ELECTRODES; O-2; REDUCTION; FUEL-CELLS; DIOXYGEN; ENZYME;
D O I
10.1246/cl.180597
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A technique for immobilizing enzyme on Au electrode was developed. The oxygen reduction reaction activity of electrochemically deposited enzymes was higher than that of physically adsorbed enzymes. Based on the electrochemical quartz crystal microbalance measurements, the higher ORR activity of electrochemically deposited enzymes could be attributed to the proper orientation of the enzymes. In addition, the electrochemically deposited enzymes exhibited high stability with respect to potential cycles.
引用
收藏
页码:1269 / 1271
页数:3
相关论文
共 50 条
  • [1] Site-Directed Immobilization of Bilirubin Oxidase for Electrocatalytic Oxygen Reduction
    Al-Lolage, Firas A.
    Bartlett, Philip N.
    Gounel, Sebastien
    Staigre, Priscilla
    Mano, Nicolas
    ACS CATALYSIS, 2019, 9 (03) : 2068 - 2078
  • [2] Bioelectrocatalytic Oxygen Reduction Reaction by Bilirubin Oxidase Adsorbed on Glassy Carbon and Edge-Plane Pyrolytic Graphite Electrodes: Effect of Redox Mediators
    Li, De-an
    Okajima, Takeyoshi
    Mao, Lanqun
    Ohsaka, Takeo
    INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, 2014, 9 (03): : 1390 - 1398
  • [3] Biosensor based on an oxygen reducing bilirubin oxidase electrode
    Goebel, G.
    Dietz, T.
    Lisdat, F.
    PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE, 2009, 1 (01): : 273 - 276
  • [4] Effective bioelectrocatalysis of bilirubin oxidase on electrochemically reduced graphene oxide
    Filip, Jaroslav
    Tkac, Jan
    ELECTROCHEMISTRY COMMUNICATIONS, 2014, 49 : 70 - 74
  • [5] Bilirubin oxidase labeling triggers an efficient signaling mechanism of oxygen reduction reaction for smart photocathodic immunoassay
    Zhang, Di
    Gu, Shiting
    Zong, Lingbo
    Hu, Ze
    Fan, Gao-Chao
    Luo, Xiliang
    SENSORS AND ACTUATORS B-CHEMICAL, 2021, 330
  • [6] Mechanistic studies of the 'blue' Cu enzyme, bilirubin oxidase, as a highly efficient electrocatalyst for the oxygen reduction reaction
    dos Santos, Luciano
    Climent, Victor
    Blanford, Christopher F.
    Armstrong, Fraser A.
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2010, 12 (42) : 13962 - 13974
  • [7] Oxygen reduction reactions of the thermostable bilirubin oxidase from Bacillus pumilus on mesoporous carbon-cryogel electrodes
    Tsujimura, Seiya
    Suraniti, Emmanuel
    Durand, Fabien
    Mano, Nicolas
    ELECTROCHIMICA ACTA, 2014, 117 : 263 - 267
  • [8] Significance of the Length of Carbon Nanotubes on the Bioelectrocatalytic Activity of Bilirubin Oxidase for Dioxygen Reduction
    So, Keisei
    Onizuka, Maki
    Komukai, Takuji
    Kitazumi, Yuki
    Shirai, Osamu
    Kano, Kenji
    ELECTROCHIMICA ACTA, 2016, 192 : 133 - 138
  • [9] Monitoring catalytic reaction of bilirubin oxidase and determination of bilirubin and bilirubin oxidase activity by capillary electrophoresis
    Zhou, XM
    Liu, JW
    Zou, X
    Chen, JJ
    ELECTROPHORESIS, 1999, 20 (09) : 1916 - 1920
  • [10] Comparison of Direct and Mediated Electron Transfer for Bilirubin Oxidase from Myrothecium Verrucaria. Effects of Inhibitors and Temperature on the Oxygen Reduction Reaction
    Antiochia, Riccarda
    Oyarzun, Diego
    Sanchez, Julio
    Tasca, Federico
    CATALYSTS, 2019, 9 (12)