Cells Cultured on Core-Shell Photonic Crystal Barcodes for Drug Screening

被引：112

作者：

Fu, Fanfan ^{[1
]}

Shang, Luoran ^{[1
]}

Zheng, Fuyin ^{[1
]}

Chen, Zhuoyue ^{[1
]}

Wang, Huan ^{[1
]}

Wang, Jie ^{[1
]}

Gu, Zhongze ^{[1
]}

Zhao, Yuanjin ^{[1
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2016年 / 8卷 / 22期

基金：

高等学校博士学科点专项科研基金; 美国国家科学基金会;

关键词：

colloidal crystal; barcode; cell spheroid-on-a-chip; drug screening; microfluidics; CIRCULATING TUMOR-CELLS; CANCER-CELLS; CAPTURE; MICROPARTICLES; MICROCAPSULES; MICROSPHERES; ARCHITECTURE; PARTICLES; COMPLEXES; HYDROGELS;

D O I：

10.1021/acsami.6b04966

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The development of effective drug screening platforms is an important task for biomedical engineering. Here, a novel methacrylated gelatin (GelMA) hydrogel-encapsulated core-shell photonic crystal (PhC) barcode particle was developed for three-dimensional cell aggregation culture and drug screening. The GelMA shells of the barcode particles enable creation of a three-dimensional extracellular matrix (ECM) microenvironment for cell adhesion and growth, while the PhC cores of the barcode particles provide stable diffraction peaks that can encode different cell spheroids during culture and distinguish their biological response during drug testing. The applicability of this cell spheroids-on-barcodes platform was investigated by testing the cytotoxic effect of tegafur (TF), a prodrug of 5-fluorouracil (5-FU), on barcode particle-loaded liver HepG2 and HCT-116 colonic tumor cell spheroids. The cytotoxicity of TF against the HCT-116 tumor cell spheroids was enhanced in systems using cocultures of HepG2 and NIH-3T3 cells, indicating the effectiveness of this multiple cell spheroids-on-barcodes platform for drug screening.

引用

页码：13840 / 13848

页数：9

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