3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

被引:54
作者
Brink, Susanne C. van den [1 ,2 ]
van Oudenaarden, Alexander [1 ,2 ]
机构
[1] Univ Med Ctr Utrecht, Utrecht, Netherlands
[2] Royal Netherlands Acad Arts & Sci, Oncode Inst, Hubrecht Inst KNAW, Utrecht, Netherlands
关键词
HUMAN EMBRYO RESEARCH; LEFT-RIGHT ASYMMETRY; SELF-ORGANIZATION; SPECIFICATION; DIFFERENTIATION; MORPHOGENESIS; MAINTENANCE; ELONGATION; GUIDELINES; PATTERNS;
D O I
10.1016/j.tcb.2021.06.007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.
引用
收藏
页码:747 / 759
页数:13
相关论文
共 83 条
  • [1] Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro
    Amadei, Gianluca
    Lau, Kasey Y. C.
    De Jonghe, Joachim
    Gantner, Carlos W.
    Sozen, Berna
    Chan, Christopher
    Zhu, Meng
    Kyprianou, Christos
    Hollfelder, Florian
    Zernicka-Goetz, Magdalena
    [J]. DEVELOPMENTAL CELL, 2021, 56 (03) : 366 - +
  • [2] Anlas K., 2020, ARXIV201101294
  • [3] Initiating head development in mouse embryos: integrating signalling and transcriptional activity
    Arkell, Ruth M.
    Tam, Patrick P. L.
    [J]. OPEN BIOLOGY, 2012, 2
  • [4] Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
    Baillie-Johnson, Peter
    van den Brink, Susanne Carina
    Balayo, Tina
    Turner, David Andrew
    Arias, Alfonso Martinez
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015, (105):
  • [5] Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids
    Beccari, Leonardo
    Moris, Naomi
    Girgin, Mehmet
    Turner, David A.
    Baillie-Johnson, Peter
    Cossy, Anne-Catherine
    Lutolf, Matthias P.
    Duboule, Denis
    Arias, Alfonso Martinez
    [J]. NATURE, 2018, 562 (7726) : 272 - +
  • [6] Geometrical confinement controls the asymmetric patterning of brachyury in cultures of pluripotent cells
    Blin, Guillaume
    Wisniewski, Darren
    Picart, Catherine
    Thery, Manuel
    Puceat, Michel
    Lowell, Sally
    [J]. DEVELOPMENT, 2018, 145 (18):
  • [7] Gastruloid Development Competence Discriminates Different States of Pluripotency
    Cermola, Federica
    D'Aniello, Cristina
    Tate, Rosarita
    De Cesare, Dario
    Martinez-Arias, Alfonso
    Minchiotti, Gabriella
    Patriarca, Eduardo Jorge
    [J]. STEM CELL REPORTS, 2021, 16 (02): : 354 - 369
  • [8] Three neural tubes in mouse embryos with mutations in the T-box gene Tbx6
    Chapman, DL
    Papaioannou, VE
    [J]. NATURE, 1998, 391 (6668) : 695 - 697
  • [9] Human embryo research, stem cell-derived embryo models and in vitro gametogenesis: Considerations leading to the revised ISSCR guidelines
    Clark, Amander T.
    Brivanlou, Ali
    Fu, Jianping
    Kato, Kazuto
    Mathews, Debra
    Niakan, Kathy K.
    Rivron, Nicolas
    Saitou, Mitinori
    Surani, Azim
    Tang, Fuchou
    Rossant, Janet
    [J]. STEM CELL REPORTS, 2021, 16 (06): : 1416 - 1424
  • [10] Currias N.M.L.P., 2020, EARLY NEURULATION RE, DOI [10.1101/2020.02.13.947655, DOI 10.1101/2020.02.13.947655]