Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains

被引：1
作者：
Bonomo, Maria Grazia [1 ]
Sico, Maria Anna [1 ]
Grieco, Simona [1 ]
Salzano, Giovanni [1 ]
机构：
[1] Univ Basilicata, Dipartimento Biol Difesa & Biotecnol Agroforestal, I-85100 Potenza, Italy
来源：
NUTRIENTS | 2009年 / 1卷 / 02期
关键词：
Fluorescent Differential Display PCR; Lactobacillus sakei; stress response;
D O I：
10.3390/nu1020210
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
Lactobacillus sakei is widely used as starter in the production process of Italian fermented sausages and its growth and survival are affected by various factors. We studied the differential expression of genome in response to different stresses by the fluorescent differential display (FDD) technique. This study resulted in the development and optimization of an innovative technique, with a high level of reproducibility and quality, which allows the identification of gene expression changes associated with different microbial behaviours under different growth conditions.
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收藏
页码：210 / 223
页数：14
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