A novel approach for increasing transformation efficiency in E. coli DH5 cells using silver nanoparticles

被引：10
作者：
Nagamani, Gorantla [1 ]
Alex, Swapna [1 ]
Soni, K. B. [1 ]
Anith, K. N. [2 ]
Viji, M. M. [3 ]
Kiran, A. G. [1 ]
机构：
[1] Coll Agr, Dept Plant Biotechnol, Thiruvananthapuram 695522, Kerala, India
[2] Coll Agr, Dept Agr Microbiol, Thiruvananthapuram 695522, Kerala, India
[3] Coll Agr, Dept Plant Physiol, Thiruvananthapuram 695522, Kerala, India
来源：
3 BIOTECH | 2019年 / 9卷 / 03期
关键词：
Silver nanoparticles; Competent cells; Bacterial transformation; pUC18; plasmid; pBR322; pCAMBIA; -Galactosidase; ESCHERICHIA-COLI; METAL NANOPARTICLES; ANTIBIOTICS; GENE;
D O I：
10.1007/s13205-019-1640-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The present study is the first report on the application of silver nanoparticles for efficient bacterial transformation. EC50 value of 100nm silver nanoparticles against E. coli DH5 cells was recorded as 4.49mgL(-1) in toxicity assay. Competency induction in E. coli DH5 cells by treatment with 100nm silver nanoparticles at a concentration of 1mgL(-1) for 60min and transformation using three plasmid vectors of different sizes, viz. pUC18, pBR322 and pCAMBIA resulted in tenfold increase in the bacterial transformation efficiency, i.e. 8.3x10(4), 8.0x10(4) and 7.9x10(4)cfung(-1) of DNA, respectively, even without heat shock compared to the conventional chemical method using 0.1M calcium chloride (2.3x10(3)cfung(-1) of DNA).
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页数：7
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