Proteomic analysis of differentially accumulated proteins during ripening and in response to 1-MCP in papaya fruit

被引：60

作者：

Angel Huerta-Ocampo, Jose ^{[1
]}

Alberto Osuna-Castro, Juan ^{[2
]}

Jareth Lino-Lopez, Gisela ^{[2
]}

Barrera-Pacheco, Alberto ^{[1
]}

Mendoza-Hernandez, Guillermo ^{[3
]}

De Leon-Rodriguez, Antonio ^{[1
]}

Barba de la Rosa, Ana Paulina ^{[1
]}

机构：

[1] IPICyT, San Luis Potosi 78216, Mexico

[2] Univ Colima Tecoman, Fac Ciencias Biol & Agr, Colima, Mexico

[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico

来源：

JOURNAL OF PROTEOMICS | 2012年 / 75卷 / 07期

关键词：

Carica papaya L; LC/ESI-MS/MS; Two-dimensional electrophoresis; Ripening; 1-Methylcyclopropene; CONTROLLED TUMOR PROTEIN; ALDO-KETO REDUCTASES; COLD-STORAGE; ARABIDOPSIS-THALIANA; GEL-ELECTROPHORESIS; ANTIOXIDANT SYSTEM; DISEASE RESISTANCE; 1-METHYLCYCLOPROPENE; STRESS; L;

D O I：

10.1016/j.jprot.2012.01.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Papaya (Carica papaya L.) is a climacteric fruit susceptible to postharvest losses due to the ethylene-induced ripening. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), has been used worldwide as a safe postharvest non-toxic agent, but the physiological and biochemical modifications induced by 1-MCP are not well understood. Using the 2-DE analysis, we report the changes in the protein profiles after 6 and 18 days of postharvest and the effect of the effect of 1-MCP treatment on fruits. Twenty seven protein spots showing differences in abundance during ripening were successfully identified by nano-LC-ESI/MS/MS. Some spots corresponded to the cell wall degrading enzymes related to fruit ripening; others were involved in oxidative damage protection, protein folding, and cell growth and survival that were induced by 1-MCP. This is the first proteomic report analyzing the effect of 1-MCP in papaya ripening. The present data will help to shed light on papaya fruit ripening process. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：2160 / 2169

页数：10

共 64 条

[51] Molecular cloning, characterization and expression of cDNA encoding translationally controlled tumor protein (TCTP) from Jatropha curcas L [J].