Proteomic analysis of differentially accumulated proteins during ripening and in response to 1-MCP in papaya fruit

被引：60
作者：
Angel Huerta-Ocampo, Jose [1 ]
Alberto Osuna-Castro, Juan [2 ]
Jareth Lino-Lopez, Gisela [2 ]
Barrera-Pacheco, Alberto [1 ]
Mendoza-Hernandez, Guillermo [3 ]
De Leon-Rodriguez, Antonio [1 ]
Barba de la Rosa, Ana Paulina [1 ]
机构：
[1] IPICyT, San Luis Potosi 78216, Mexico
[2] Univ Colima Tecoman, Fac Ciencias Biol & Agr, Colima, Mexico
[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico
来源：
JOURNAL OF PROTEOMICS | 2012年 / 75卷 / 07期
关键词：
Carica papaya L; LC/ESI-MS/MS; Two-dimensional electrophoresis; Ripening; 1-Methylcyclopropene; CONTROLLED TUMOR PROTEIN; ALDO-KETO REDUCTASES; COLD-STORAGE; ARABIDOPSIS-THALIANA; GEL-ELECTROPHORESIS; ANTIOXIDANT SYSTEM; DISEASE RESISTANCE; 1-METHYLCYCLOPROPENE; STRESS; L;
D O I：
10.1016/j.jprot.2012.01.015
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Papaya (Carica papaya L.) is a climacteric fruit susceptible to postharvest losses due to the ethylene-induced ripening. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), has been used worldwide as a safe postharvest non-toxic agent, but the physiological and biochemical modifications induced by 1-MCP are not well understood. Using the 2-DE analysis, we report the changes in the protein profiles after 6 and 18 days of postharvest and the effect of the effect of 1-MCP treatment on fruits. Twenty seven protein spots showing differences in abundance during ripening were successfully identified by nano-LC-ESI/MS/MS. Some spots corresponded to the cell wall degrading enzymes related to fruit ripening; others were involved in oxidative damage protection, protein folding, and cell growth and survival that were induced by 1-MCP. This is the first proteomic report analyzing the effect of 1-MCP in papaya ripening. The present data will help to shed light on papaya fruit ripening process. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：2160 / 2169
页数：10
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