MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers

被引：76

作者：

Martinelli-Boneschi, Filippo ^{[1
,2
]}

Fenoglio, Chiara ^{[3
]}

Brambilla, Paola ^{[1
,2
]}

Sorosina, Melissa ^{[1
,2
]}

Giacalone, Giacomo ^{[1
,2
]}

Esposito, Federica ^{[1
,2
]}

Serpente, Maria ^{[3
]}

Cantoni, Claudia ^{[3
]}

Ridolfi, Elisa ^{[3
]}

Rodegher, Mariaemma ^{[1
,2
]}

Moiola, Lucia ^{[1
,2
]}

Colombo, Bruno ^{[1
,2
]}

De Riz, Milena ^{[3
]}

Martinelli, Vittorio ^{[1
,2
]}

Scarpini, Elio ^{[3
]}

Comi, Giancarlo ^{[1
,2
]}

Galimberti, Daniela ^{[3
]}

机构：

[1] Ist Sci San Raffaele, Inst Expt Neurol INSPE, I-20132 Milan, Italy

[2] Ist Sci San Raffaele, Dept Neurol, Div Neurosci, I-20132 Milan, Italy

[3] Univ Milan, Dept Neurol Sci, Dino Ferrari Ctr, Fdn Ca Granda,IRCCS Osped Maggiore Policlin, I-20122 Milan, Italy

来源：

NEUROSCIENCE LETTERS | 2012年 / 508卷 / 01期

关键词：

Multiple sclerosis; MicroRNA; Gene expression; DIAGNOSTIC-CRITERIA; HEALTHY CONTROLS; DISEASE; TRANSCRIPTION; PATHWAYS; LESIONS; CELLS;

D O I：

10.1016/j.neulet.2011.11.006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Identification of novel targets and biomarkers, such as microRNAs, is extremely helpful to understand the pathogenetic mechanisms in a disease like multiple sclerosis (MS). We tested the expression profile of 1145 microRNAs in peripheral blood mononuclear cells (PBMCs) of 19 MS patients and 14 controls, and we further explored their function by performing a whole-genome mRNA profiling in same subjects and using bioinformatic prediction tool. A total of 104 miRNAs have been identified as deregulated in MS patients: 2/10 which ranked highest (let-7g and miR-150) have been validated in a replication sample, leading to the identification of putative target genes. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：4 / 8

页数：5

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