Determination of Organophosphorus Pesticides Based on Biotin-Avidin Enzyme Linked Immunosorbent Assay

被引：15

作者：

Wang Lei ^{[1
]}

Zhang Li-Jie ^{[1
]}

Lue Wei ^{[1
]}

Han Shi-He ^{[1
,2
]}

Zhang Fu-Kai ^{[1
]}

Pan Jia-Rong ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Agro Food Sci & Technol, Key Lab Agro Food Proc & Qual Control, Minist Agr, Beijing 10019, Peoples R China

[2] Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Xian 710062, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2011年 / 39卷 / 03期

关键词：

Pesticides; O; O-Diethyl organophosphorus; Biotin-avidin amplified enzyme linked immunosorbent assay; IMMUNOASSAY; ELISA;

D O I：

10.1016/S1872-2040(10)60425-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A multienzyme system was generated by the specific reaction between biotin and avidin. Based on the system, a biotin-avidin amplified enzyme-linked immunosorbent assay ( BA-ELISA) method was developed and optimized for the determination of O,O-diethyl organophosphorus pesticides. Under the optimized experiment conditions, a good linear relationship between the concentrations of O, O-diethyl organophosphorus pesticides and inhibition ratio in the range of 0. 1-1. 0 X 10(4)mu g/L was obtained. The regression equation for the determination of diethylphosphonoacetic acid (DPA) was y=0. 1168 1gC(DPA) +0. 6259 (r = 0. 9889), 1050 and the detection limit were 34 and 0.0248 mu g/L, respectively. Compared with the ELISA, the sensitivity and detecting range were significantly improved while the cross-reaction remained unchanged. The recovery of the spiked samples was in the range from 70. 1% to 117. 8%, basically meeting the requirement of trace analysis. (O,O-diethyl organophosphorus pesticides.

引用

页码：346 / 350

页数：5

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