Evaluation of citrus somatic hybrids for tolerance to Phytophthora nicotianae and citrus tristeza virus

被引：17
作者：
Alves Mourao Filho, Francisco de Assis [1 ]
Pio, Rafael [1 ]
Januzzi Mendes, Beatriz Madalena [2 ]
de Azevedo, Fernando Alves [1 ]
Schinor, Evandro Henrique [1 ]
Entelmann, Fabio Albuquerque [1 ]
Rodrigues Alves, Andre Siqueira [1 ]
Cantuarias-Aviles, Tatiana Eugenia [1 ]
机构：
[1] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Dept Prod Vegetal, BR-13418900 Piracicaba, SP, Brazil
[2] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba, SP, Brazil
来源：
SCIENTIA HORTICULTURAE | 2008年 / 115卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
CTV; disease resistance; protoplast fusion; rootstock; trunk rot; HYBRIDIZATION; IMPROVEMENT; RESISTANCE; ROOTSTOCK;
D O I：
10.1016/j.scienta.2007.10.004
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Somatic hybridization is a biotechnology tool that can be used in citrus breeding programs to produce somatic hybrids with the complete genetic combination of both parents. The goal of this work was to test the reaction of citrus somatic hybrids that may be useful as rootstocks to trunk and root infections caused by Phytophthora nicotianae van Breda de Haan (P parasitica Dastur) and to citrus tristeza virus (CTV). The somatic hybrids evaluated were 'Caipira' sweet orange (Citrus sinensis L. Osbeck) + 'Rangpur' lime (C. limonia Osbeck), 'Caipira' sweet orange + 'Cleopatra' mandarin (C. reshni hort. ex Tanaka), 'Caipira' sweet orange + 'Volkamer' lemon (C. volkameriana V Ten. & Pasq.), 'Caipira' sweet orange + rough lemon (C. jambhiri Lush.), 'Cleopatra' mandarin + 'Volkamer' lemon, 'Cleopatra' mandarin + sour orange (C. aurantium L.), 'Rangpur' lime + 'Sunki' mandarin (C. sunki (Hayata) hort. ex Tanaka), 'Ruby Blood' sweet orange (C. sinensis L. Osbeck) + 'Volkamer' lemon, 'Rohde Red' sweet orange (C. sinensis L. Osbeck) + 'Volkamer' lemon, and 'Valencia' sweet orange + Fortunella obovata hort. ex Tanaka. For P. nicotianae trunk and root infection assays, plants of the somatic hybrids, obtained from 9-month semi-hardwood cuttings, were evaluated and compared with diploid citrus rootstock cultivars after mycelia inoculation in the trunk or spore infestation in the substrate, respectively. 'Cleopatra' mandarin + sour orange, 'Rangpur' lime + 'Sunki' mandarin, 'Cleopatra' mandarin + 'Volkamer' lemon, 'Ruby Blood' sweet orange + 'Volkamer' lemon, 'Rohde Red' sweet orange + 'Volkamer' lemon, and 'Caipira' sweet orange + 'Volkamer' lemon had less trunk rot occurrence, whereas the somatic hybrids 'Cleopatra' mandarin + 'Volkamer' lemon, 'Cleopatra' mandarin + sour orange, 'Caipira' sweet orange + 'Volkamer' lemon, and 'Caipira' sweet orange + 'Rangpur' lime were tolerant to root rot. For CTV assays, plants of the somatic hybrids along with tolerant and intolerant rootstocks were budded with a mild strain CTV-infected or healthy 'Valencia' sweet orange budwood. Differences in average scion shoot length indicated that the hybrids 'Cleopatra' mandarin + sour orange and 'Valencia' sweet orange + Fortunella obovata were intolerant to CTV (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：301 / 308
页数：8
相关论文
共 34 条
  • [1] THE CONTINUOUS CHALLENGE OF CITRUS TRISTEZA VIRUS CONTROL
    BARJOSEPH, M
    MARCUS, R
    LEE, RF
    [J]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 1989, 27 : 291 - 316
  • [2] Benedito V. A., 2000, Scientia Agricola, V57, P33, DOI 10.1590/S0103-90162000000100007
  • [3] BENNETT C. W., 1949, JOUR AGRIC RES, V78, P207
  • [4] BROADBENT P, 1992, P INT SOC CITR, V2, P758
  • [5] Somatic hybridization between Citrus sinensis (L.) Osbeck and C-grandis (L.) Osbeck
    Calixto, MC
    Mourao, FDA
    Mendes, BMJ
    Vieira, MLC
    [J]. PESQUISA AGROPECUARIA BRASILEIRA, 2004, 39 (07) : 721 - 724
  • [6] CARLOS EF, 1997, PORTAENXERTOS CITRIC
  • [7] COSTA A. S., 1954, PROC FLORIDA STATE HORT SOC, V67, P26
  • [8] Costa A.S., 1980, Plant Disease, V64, P538, DOI [DOI 10.1094/PD-64-538, 10.1094/PD-64-538]
  • [9] Somatic hybridisation for improvement of citrus rootstock: production of five new combinations with potential for improved disease resistance
    Costa, MAPD
    Mendes, BMJ
    Mourao, FAA
    [J]. AUSTRALIAN JOURNAL OF EXPERIMENTAL AGRICULTURE, 2003, 43 (09): : 1151 - 1156
  • [10] Da Gloria FJM, 2000, PESQUI AGROPECU BRAS, V35, P727, DOI 10.1590/S0100-204X2000000400008
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