Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

被引：15

作者：

Gustafson, Tiffany P. ^{[1
]}

Metzel, Greg A. ^{[1
]}

Kutateladze, Andrei G. ^{[1
]}

机构：

[1] Univ Denver, Dept Chem & Biochem, Denver, CO 80208 USA

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2011年 / 9卷 / 13期

关键词：

BIO-BARCODE ASSAY; SIGNAL AMPLIFICATION; PROTEIN;

D O I：

10.1039/c1ob05289f

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Amplified fluorescence quenching methodology based on massive autocatalytic photo-unmasking of a dual function sensitizer-quencher is developed and adopted for photoassisted ultra-sensitive detection of molecular recognition events. The resulting binding assay, based on a molecular recognition-triggered photo-amplified cascade with concomitant decrease of fluorescence is validated with the biotin-avidin pair, achieving attomolar detection.

引用

页码：4752 / 4755

页数：4

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