Trypanocidal activity of free and nanoencapsulated curcumin on Trypanosoma evansi

被引：25

作者：

Gressler, L. T. ^{[1
]}

Oliveira, C. B. ^{[1
]}

Coradini, K. ^{[2
]}

Dalla Rosa, L. ^{[1
]}

Grando, T. H. ^{[1
]}

Baldissera, M. D. ^{[1
]}

Zimmermann, C. E. ^{[1
]}

Da Silva, A. S. ^{[3
]}

Almeida, T. C. ^{[4
]}

Hermes, C. L. ^{[4
]}

Wolkmer, P. ^{[5
]}

Silva, C. B. ^{[6
]}

Moreira, K. L. S. ^{[7
]}

Beck, R. C. R. ^{[2
]}

Moresco, R. N. ^{[4
]}

Da Veiga, M. L. ^{[5
]}

Stefani, L. M. ^{[3
]}

Monteiro, S. G. ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Dept Microbiol & Parasitol, BR-97119900 Santa Maria, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Farmaceut, Fac Farm, Porto Alegre, RS, Brazil

[3] Univ Estado Santa Catarina UDESC, Dept Anim Sci, Chapeco, Brazil

[4] Univ Fed Santa Maria, Dept Clin & Toxicol Anal, Santa Maria, RS, Brazil

[5] Univ Cruz Alta UNICRUZ, Cruz Alta, Brazil

[6] Univ Fed Santa Maria, Dept Small Anim, Santa Maria, RS, Brazil

[7] Univ Fed Santa Maria, Dept Morphol, Santa Maria, RS, Brazil

来源：

PARASITOLOGY | 2015年 / 142卷 / 03期

关键词：

trypanosomes; curcumin; nanoparticles; oxidative stress; LIPID-CORE NANOCAPSULES; ANTIOXIDANT; EQUIPERDUM; OXIDATION; TOXICITY; EFFICACY; RELEASE; SYSTEM; FORMS;

D O I：

10.1017/S0031182014001292

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

This study aimed to evaluate in vitro and in vivo trypanocidal activity of free and nanoencapsulated curcumin against Trypanosoma evansi. In vitro efficacy of free curcumin (CURC) and curcumin-loaded in lipid-core nanocapsules (C-LNCs) was evaluated to verify their lethal effect on T. evansi. To perform the in vivo tests, T. evansi-infected animals were treated with CURC (10 and 100mgkg(-1), intraperitoneally [i.p.]) and C-LNCs (10mgkg(-1), i.p.) during 6 days, with the results showing that these treatments significantly attenuated the parasitaemia. Infected untreated rats showed protein peroxidation and an increase of nitrites/nitrates, whereas animals treated with curcumin showed a reduction on these variables. As a result, the activity of antioxidant enzymes (superoxide dismutase and catalase) differs between groups (P<005). Infected animals and treated with CURC exhibited a reduction in the levels of alanine aminotransferase and creatinine, when compared with the positive control group. The use of curcumin in vitro resulted in a better parasitaemia control, an antioxidant activity and a protective effect on liver and kidney functions of T. evansi-infected adult male Wistar rats.

引用

页码：439 / 448

页数：10

共 53 条

[1] Antioxidant and radical scavenging properties of curcumin [J].

Ak, Tuba ;

Gulcin, Ilhami .

CHEMICO-BIOLOGICAL INTERACTIONS, 2008, 174 (01) :27-37

[2] Bioavailability of curcumin: Problems and promises [J].

Anand, Preetha ;

Kunnumakkara, Ajaikumar B. ;

Newman, Robert A. ;

Aggarwal, Bharat B. .

MOLECULAR PHARMACEUTICS, 2007, 4 (06) :807-818

[3] Trypanocidal activity of the essential oils in their conventional and nanoemulsion forms: In vitro tests [J].

Baldissera, Matheus D. ;

Da Silva, Aleksandro S. ;

Oliveira, Camila B. ;

Zimmermann, Carine E. P. ;

Vaucher, Rodrigo A. ;

Santos, Roberto C. V. ;

Rech, Virginia C. ;

Tonin, Alexandre A. ;

Giongo, Janice L. ;

Mattos, Cristiane B. ;

Koester, Leticia ;

Santurio, Janio M. ;

Monteiro, Silvia G. .

EXPERIMENTAL PARASITOLOGY, 2013, 134 (03) :356-361

[4] CULTIVATION IN A SEMI-DEFINED MEDIUM OF ANIMAL INFECTIVE FORMS OF TRYPANOSOMA-BRUCEI, TRYPANOSOMA-EQUIPERDUM, TRYPANOSOMA-EVANSI, TRYPANOSOMA-RHODESIENSE AND T-GAMBIENSE [J].

BALTZ, T ;

BALTZ, D ;

GIROUD, C ;

CROCKETT, J .

EMBO JOURNAL, 1985, 4 (05) :1273-1277

[5] Disseminated central nervous system disease caused by Trypanosoma evansi in a horse [J].

Berlin, Dalia ;

Loeb, Emmanuel ;

Baneth, Gad .

VETERINARY PARASITOLOGY, 2009, 161 (3-4) :316-319

[6] Indomethacin-loaded lipid-core nanocapsules reduce the damage triggered by Aβ1-42 in Alzheimer's disease models [J].

Bernardi, Andressa ;

Frozza, Rudimar L. ;

Meneghetti, Andre ;

Hoppe, Juliana B. ;

Battastini, Ana Maria O. ;

Pohlmann, Adriana R. ;

Guterres, Silvia S. ;

Salbego, Christianne G. .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 :4927-4942

[7] Indomethacin-loaded nanocapsules treatment reduces in vivo glioblastoma growth in a rat glioma model [J].

Bernardi, Andressa ;

Braganhol, Elizandra ;

Jaeger, Eliezer ;

Figueiro, Fabricio ;

Edelweiss, Maria Isabel ;

Pohlmann, Adriana R. ;

Guterres, Silvia S. ;

Battastini, Ana M. O. .

CANCER LETTERS, 2009, 281 (01) :53-63

[8]

Brandao L. P., 2002, Clinica Veterinaria, V7, P23

[9] Trypanosoma evansi and T-equiperdum: distribution, biology, treatment and phylogenetic relationship (a review) [J].

Brun, R ;

Hecker, H ;

Lun, ZR .

VETERINARY PARASITOLOGY, 1998, 79 (02) :95-107

[10]

Colpo Cristina Braccini, 2005, Cienc. Rural, V35, P717, DOI 10.1590/S0103-84782005000300038

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