Impairment of the Ubiquitin-Proteasome Pathway in RPE Alters the Expression of Inflammation Related Genes

被引：21

作者：

Liu, Zhenzhen ^{[1
]}

Qin, Tingyu ^{[1
]}

Zhou, Jilin ^{[2
]}

Taylor, Allen ^{[1
]}

Sparrow, Janet R. ^{[2
]}

Shang, Fu ^{[1
]}

机构：

[1] Tufts Univ, Lab Nutr & Vis Res, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA

[2] Columbia Univ, Dept Ophthalmol, New York, NY 10027 USA

来源：

RETINAL DEGENERATIVE DISEASES: MECHANISMS AND EXPERIMENTAL THERAPY | 2014年 / 801卷

关键词：

Age-related macular degeneration; Inflammation; Ubiquitin; Proteasome; Retinal pigment epithelial cells; IL-6; IL-8; MCP-1; Complement factor H; PIGMENT EPITHELIAL-CELLS; FACTOR-H POLYMORPHISM; NF-KAPPA-B; C-REACTIVE PROTEIN; MACULAR DEGENERATION; OXIDATIVE STRESS; CONJUGATING ENZYMES; PARKINSONS-DISEASE; ENDOTHELIAL-CELLS; PATHOGENESIS;

D O I：

10.1007/978-1-4614-3209-8_31

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ubiquitin-proteasome pathway (UPP) plays an important role in regulating gene expression. Retinal pigment epithelial cells (RPE) are a major source of ocular inflammatory cytokines. In this work we determined the relationship between impairment of the UPP and expression of inflammation-related factors. The UPP could be impaired by oxidative stress or chemical inhibition. Impairment of the UPP in RPE increased the expression of several inflammatory cytokines, such as IL-6 and IL-8. However, the expression of monocyte chemoattractant protein-1 (MCP-1) and complement factor H (CFH) and was reduced upon impairment of the UPP. These data suggest that impairment of the UPP in RPE may be one of the causes of retinal inflammation and abnormal functions of monocyte and the complement system during the pathogenesis of age-related macular degeneration.

引用

页码：237 / 250

页数：14

共 68 条

[1] An animal model of age-related macular degeneration in senescent Ccl-2-or Ccr-2-deficient mice [J].

Ambati, J ;

Anand, A ;

Fernandez, S ;

Sakurai, E ;

Lynn, BC ;

Kuziel, WA ;

Rollins, BJ ;

Ambati, BK .

NATURE MEDICINE, 2003, 9 (11) :1390-1397

[2] Age-related macular degeneration: Etiology, pathogenesis, and therapeutic strategies [J].

Ambati, J ;

Ambati, BK ;

Yoo, SH ;

Ianchulev, S ;

Adamis, AP .

SURVEY OF OPHTHALMOLOGY, 2003, 48 (03) :257-293

[3] Perspective - A role for local inflammation in the formation of drusen in the aging eye [J].

Anderson, DH ;

Mullins, RF ;

Hageman, GS ;

Johnson, LV .

AMERICAN JOURNAL OF OPHTHALMOLOGY, 2002, 134 (03) :411-431

[4] The pivotal role of the complement system in aging and age-related macular degeneration: Hypothesis re-visited [J].

Anderson, Don H. ;

Radeke, Monte J. ;

Gallo, Natasha B. ;

Chapin, Ethan A. ;

Johnson, Patrick T. ;

Curletti, Christy R. ;

Hancox, Lisa S. ;

Hu, Jane ;

Ebright, Jessica N. ;

Malek, Goldis ;

Hauser, Michael A. ;

Rickman, Catherine Bowes ;

Bok, Dean ;

Hageman, Gregory S. ;

Johnson, Lincoln V. .

PROGRESS IN RETINAL AND EYE RESEARCH, 2010, 29 (02) :95-112

[5] Oxidative modification and inactivation of the proteasome during coronary occlusion/reperfusion [J].

Bulteau, AL ;

Lundberg, KC ;

Humphries, KM ;

Sadek, HA ;

Szweda, PA ;

Friguet, B ;

Szweda, LI .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (32) :30057-30063

[6] Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells [J].

Caballero, M ;

Liton, PB ;

Epstein, DL ;

Gonzalez, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 308 (02) :346-352

[7] Nutritional antioxidants and age-related cataract and maculopathy [J].

Chiu, Chung-Jung ;

Taylor, Allen .

EXPERIMENTAL EYE RESEARCH, 2007, 84 (02) :229-245

[8]

Chiu CJ, 2009, NUTR HEALTH SER, P501, DOI 10.1007/978-1-60327-542-2_21

[9] The ubiquitin proteolytic system and pathogenesis of human diseases: a novel platform for mechanism-based drug targeting [J].

Ciechanover, A .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2003, 31 :474-481

[10] Drusen proteome analysis: An approach to the etiology of age-related macular degeneration [J].

Crabb, JW ;

Miyagi, M ;

Gu, XR ;

Shadrach, K ;

West, KA ;

Sakaguchi, H ;

Kamei, M ;

Hasan, A ;

Yan, L ;

Rayborn, ME ;

Salomon, RG ;

Hollyfield, JG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14682-14687

← 1 2 3 4 5 6 7 →