Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection

被引：145
作者：
Lau, AJ
Woo, SO
Koh, HL
机构：
[1] Natl Univ Singapore, Fac Sci, Dept Pharm, Singapore 117543, Singapore
[2] Hlth Sci Author, Ctr Analyt Sci, Ind Hlth Lab, Singapore 169078, Singapore
来源：
JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1011卷 / 1-2期
关键词：
Panax notoginseng; pharmaceutical analysis; plant materials; saponins; ginsenosides; notoginsenosides;
D O I：
10.1016/S0021-9673(03)01135-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A reversed-phase high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of six saponins (notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rd) in raw and steamed Panax notoginseng. Linearity (r(2)>0.9988), intra- and inter-day precision (RSD<4%), limit of detection (0.008-0.013 mg/ml), limit of quantification (0.027-0.042 mg/ml) of the saponins were determined. The method was successfully applied to 11 pairs of raw and steamed P. notoginseng products. Three products showed discrepancies between their labelled claims (raw or steamed) and the results of analysis. This new, simple and reliable method could be used in the quality control of raw and steamed P. notoginseng. (C) 2003 Elsevier B.V. All rights reserved.
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页码：77 / 87
页数：11
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