Nanoscale resolution in the focal plane of an optical microscope

被引：348

作者：

Westphal, V ^{[1
]}

Hell, SW ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Nanobiophoton, D-37070 Gottingen, Germany

来源：

PHYSICAL REVIEW LETTERS | 2005年 / 94卷 / 14期

关键词：

D O I：

10.1103/PhysRevLett.94.143903

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down to 16 nm in the focal plane, corresponding to about 1/50 of the employed wavelength. The optical bandwidth expansion by nearly an order of magnitude is realized by a saturated depletion through stimulated emission of the molecular fluorescent state. We demonstrate that en route to the molecular scale, the resolving power increases with the square root of the saturation level, which constitutes a new law regarding the resolution of an emerging class of far-field light microscopes that are not limited by diffraction.

引用

页数：4

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