Enzyme-linked immunosorbent serum assays (ELISAs) for rat and human N-terminal pro-peptide of collagen type I (PINP) - Assessment of corresponding epitopes

被引：54
作者：
Leeming, D. J. [1 ,3 ]
Larsen, D. V. [1 ]
Zhang, C. [2 ]
Hi, Y. [2 ]
Veidal, S. S. [1 ]
Nielsen, R. H. [1 ]
Henriksen, K. [1 ]
Zheng, Q. [2 ]
Barkholt, V. [3 ]
Riis, B. J. [4 ]
Byrjalsen, I. [1 ]
Qvist, P. [1 ]
Karsdal, M. A. [1 ]
机构：
[1] Nord Biosci AS, DK-2730 Herlev, Denmark
[2] Nord Biosci Beijing, Beijing, Peoples R China
[3] Tech Univ Denmark, Dept Syst Biol, Lyngby, Denmark
[4] Nord Biosci Clin Studies AS, Ballerup, Denmark
来源：
CLINICAL BIOCHEMISTRY | 2010年 / 43卷 / 15期
关键词：
Biochemical markers; Bone formation; PINP; Translational science; Ibandronate; OVX; BDL; PROCOLLAGEN TYPE-I; BIOCHEMICAL MARKERS; BONE TURNOVER; POSTMENOPAUSAL WOMEN; ALKALINE-PHOSPHATASE; SALMON-CALCITONIN; PROPEPTIDE; IMMUNOASSAY; ALENDRONATE; PROSTATE;
D O I：
10.1016/j.clinbiochem.2010.07.025
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: The present study describes two newly developed N-terminal pro-peptides of collagen type I (PINP) competitive enzyme-linked immunosorbent assays (ELISAs) for the assessment of corresponding PINP epitopes in the rat- and human species. Methods: Monoclonal antibodies were raised against corresponding rat and human PINP sequences and competitive assays were developed for each species. They were evaluated in relevant pre-clinical or clinical studies. Results: The antibody characterizations indicated that PINP indeed was recognized. Technical robust assays were obtained. Rat PINP and tALP showed similar patterns in the gold standard osteoporosis rat ovariectomized (OVX) model. No liver contribution was observed in the liver fibrosis rat bile duct ligation model (BDL). In an osteoporosis study, the human serum PINP levels were significantly decreased after ibandronate treatment compared to placebo. Conclusions: The two corresponding PINP assays were specific and these bone turnover markers may improve translational science for the evaluation for bone-related diseases. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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页码：1249 / 1256
页数：8
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