Smart Spectrophotometric Method Development for Simultaneous Estimation of Antidiabetic Drugs in Formulations

被引:7
作者
Attimarad, Mahesh [1 ]
Venugopala, Katharigatta Narayanaswamy [1 ,2 ]
Shafi, Sheeba [3 ]
Balgoname, Abdulmalek Ahmed [1 ]
Altaysan, Abdulrahman Ibrahim [1 ]
机构
[1] King Faisal Univ, Coll Clin Pharm, Dept Pharmaceut Sci, Al Hufuf, Al Ahsa, Saudi Arabia
[2] Durban Univ Technol, Fac Appl Sci, Dept Biotechnol & Food Sci, Durban, South Africa
[3] King Faisal Univ, Coll Appl Med Sci, Dept Nursing, Al Hasa, Saudi Arabia
关键词
Antidiabetics; Vildagliptin; Remogliflozin; Derivative spectrophotometry; Formulation; Validation; VILDAGLIPTIN; METFORMIN; VALIDATION;
D O I
10.5530/ijper.56.1.26
中图分类号
G40 [教育学];
学科分类号
040101 ; 120403 ;
摘要
Background: An anti-diabetic formulation consisting of vildagliptin and remogliflozin was prescribed for better glycemic control. In the present study a simple, rapid derivative spectrophotometric methods were evolved to analyze these two analytes from the formulations. Methods: Two processed UV spectrophotometric methods were established by measuring the peak amplitude at zero-crossing of second derivative spectra of analytes. The second procedure comprehends the generation of zero - order spectra from the mixture of analyte spectra by division and multiplication by the pure analyte spectra to remove the effect of one of the analytes. Results: Both methods showed linearity concentrations in the range of 2-75 mu g/ml for RGF and 2-50 mu g/ml for VGT. The low LOD and LOQ found for RGF and VGT by both methods indicated the good sensitivity of the methods. The mean percentage recovery was 98.60 % and 100.78%, for RGF and 98.81 % and 99.15 % for VGT, with low percent relative error. The % RSD for intra and inter-day precision was less than +/- 2%. Finally, the planned methods were employed for the assay of the VGT and RGF from the medicine and the outcomes were matched with the reported methods. Conclusion: The assay results of the formulation were in agreement with the concentration of labeled amount and no significant difference was observed in the results when compared to the reported method. Hence, the anticipated procedures could be applied for the routine quality control of formulations consisting of VGT and
引用
收藏
页码:224 / 231
页数:8
相关论文
共 31 条
[1]   Simultaneous HPLC determination of vildagliptin, ampicillin, sulbactam and metronidazole in pharmaceutical dosage forms and human urine [J].
Abdel Hady, Khaled K. ;
Abdel Salam, Randa A. ;
Hadad, Ghada M. ;
Abdel Hameed, Eman A. .
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY, 2021, 18 (03) :729-738
[2]   Validation of different spectrophotometric methods for determination of vildagliptin and metformin in binary mixture [J].
Abdel-Ghany, Maha F. ;
Abdel-Aziz, Omar ;
Ayad, Miriam F. ;
Tadros, Mariam M. .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2014, 125 :175-182
[3]  
Ahsan S, 2021, Endocrine Practice, V27, pS62
[4]   UPLC-ESI/Q-TOF MS/MS Method for Determination of Vildagliptin and its Organic Impurities [J].
Alves Giordani, Camila Ferrazza ;
Campanharo, Sarah ;
Wingert, Nathalie Ribeiro ;
Bueno, Livia Maronesi ;
Manoel, Joanna Wittckind ;
Garcia, Cassia Virginia ;
Volpato, Nadia Maria ;
Iop, Gabrielle Dineck ;
Mello, Paola de Azevedo ;
de Moraes Flores, Erico Marlon ;
Scherman Schapoval, Elfrides Eva ;
Steppe, Martin .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2020, 58 (08) :718-725
[5]   Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness [J].
Attimarad, Mahesh ;
Nair, Anroop B. ;
Sreeharsha, Nagaraja ;
Al-Dhubiab, Bandar E. ;
Venugopala, Katharigatta N. ;
Shinu, Pottathil .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2021, 18 (02) :1-16
[6]   Development and Validation of Rapid RP-HPLC and Green Second-Derivative UV Spectroscopic Methods for Simultaneous Quantification of Metformin and Remogliflozin in Formulation Using Experimental Design [J].
Attimarad, Mahesh ;
Elgorashe, Rafea Elamin Elgack ;
Subramaniam, Rajasekaran ;
Islam, Mohammed Monirul ;
Venugopala, Katharigatta N. ;
Nagaraja, Sreeharsha ;
Balgoname, Abdulmalek Ahmed .
SEPARATIONS, 2020, 7 (04) :1-20
[7]   Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations [J].
Attimarad, Mahesh ;
Chohan, Muhammad Shahzad ;
Balgoname, Abdulmalek Ahmed .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2019, 16 (07)
[8]   Development of a rapid reversed phase-high performance liquid chromatography method for simultaneous determination of metformin and vildagliptin in formulation and human plasma [J].
Attimarad, Mahesh ;
Nagaraja, Sree Harsha ;
Aldhubaib, Bandar E. ;
Al-Najjar, Ahmed .
JOURNAL OF YOUNG PHARMACISTS, 2014, 6 (04) :40-46
[9]  
Bhatkar T., 2020, INT J PHARM RES, V12, DOI [10.31838/ijpr/2020.12.04.026, DOI 10.31838/IJPR/2020.12.04.026]
[10]   Manipulating Ratio Spectra for the Spectrophotometric Analysis of Diclofenac Sodium and Pantoprazole Sodium in Laboratory Mixtures and Tablet Formulation [J].
Bhatt, Nejal M. ;
Chavada, Vijay D. ;
Sanyal, Mallika ;
Shrivastav, Pranav S. .
SCIENTIFIC WORLD JOURNAL, 2014,