Single molecule biochemistry using optical tweezers

被引：15
|
作者：
Mehta, AD [1 ]
Pullen, KA [1 ]
Spudich, JA [1 ]
机构：
[1] Stanford Univ, Dept Biochem, Ctr Med, Stanford, CA 94305 USA
来源：
FEBS LETTERS | 1998年 / 430卷 / 1-2期
关键词：
optical trap; single molecule; myosin; kinesin; titin;
D O I：
10.1016/S0014-5793(98)00594-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of optical trapping to create extremely compliant mechanical probes has ushered in a new field of biological inquiry, the mechanical and kinetic study of proteins at the single molecule level, This review focuses on three examples of such study and includes methods of extracting parameters of interest from the raw data such experiments generate. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：23 / 27
页数：5
相关论文
共 50 条
  • [41] Calibrating optical tweezers with Bayesian inference
    Richly, Maximilian U.
    Tuerkcan, Silvan
    Le Gall, Antoine
    Fiszman, Nicolas
    Masson, Jean-Baptiste
    Westbrook, Nathalie
    Perronet, Karen
    Alexandrou, Antigoni
    OPTICS EXPRESS, 2013, 21 (25): : 31578 - 31590
  • [42] Probing Myosin Ensemble Mechanics in Actin Filament Bundles using Optical Tweezers
    Al Azzam, Omayma
    Watts, Janie C.
    Reynolds, Justin E.
    Davis, Juliana E.
    Reinemann, Dana N.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2022, (183):
  • [43] Step Sizes and Rate Constants of Single-headed Cytoplasmic Dynein Measured with Optical Tweezers
    Kinoshita, Yoshimi
    Kambara, Taketoshi
    Nishikawa, Kaori
    Kaya, Motoshi
    Higuchi, Hideo
    SCIENTIFIC REPORTS, 2018, 8
  • [44] Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore Arrays
    McNally, Ben
    Singer, Alon
    Yu, Zhiliang
    Sun, Yingjie
    Weng, Zhiping
    Meller, Amit
    NANO LETTERS, 2010, 10 (06) : 2237 - 2244
  • [45] Single molecule dynamics of Polyproline by using AFM
    Tamamushi, Hironori
    Kawakami, Masaru
    Furukawa, Hidemitsu
    NANOSENSORS, BIOSENSORS, INFO-TECH SENSORS AND 3D SYSTEMS 2017, 2017, 10167
  • [46] High-sensitivity Single Molecule Fluorescence Detection Using Scanning Single-Molecule Counting
    Yamaguchi, Mitsushiro
    Tanabe, Tetsuya
    Nakata, Hidetaka
    Hanashi, Takuya
    Nishikawa, Kazutaka
    Hori, Kunio
    Kondo, Seiji
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIV, 2014, 8948
  • [47] Accurate measurement of force and displacement with optical tweezers using DNA molecules as metrology standards
    delToro, Damian
    Smith, Douglas E.
    APPLIED PHYSICS LETTERS, 2014, 104 (14)
  • [48] Measuring force generation within reconstituted microtubule bundle assemblies using optical tweezers
    Al Azzam, Omayma
    Trussell, Cameron Lee
    Reinemann, Dana N.
    CYTOSKELETON, 2021, 78 (03) : 111 - 125
  • [49] Actin and myosin regulate cytoplasm stiffness in plant cells: a study using optical tweezers
    van der Honing, Hannie S.
    de Ruijter, Norbert C. A.
    Emons, Anne Mie C.
    Ketelaar, Tijs
    NEW PHYTOLOGIST, 2010, 185 (01) : 90 - 102
  • [50] Controlled Mechanical Motions of Microparticles in Optical Tweezers
    Liu, Jing
    Li, Zhiyuan
    MICROMACHINES, 2018, 9 (05)
12345