F-BOX and oleosin: additional target genes for future metabolic engineering in tung trees?

被引：16

作者：

Chen, Yicun ^{[1
]}

Zhou, Guan ^{[1
]}

Wang, Yangdong ^{[1
]}

Xu, Lingna ^{[1
]}

机构：

[1] Chinese Acad Foresty, Res Inst Subtrop Foresty, Fuyang 311400, Peoples R China

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2010年 / 32卷 / 03期

关键词：

Tung oil; cDNA library; Expressed sequence tags; DGAT2; OIL;

D O I：

10.1016/j.indcrop.2010.06.009

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Tung oil, produced from seeds of the rung tree (Vernicia fordii), is a valuable industrial oil. Genetic engineering can improve the oil content, making it more suitable for use in biodiesel, and the pivotal role of DGAT2 in triacylglycerol synthesis has been extensively studied. Our data indicated, however, that DGAT2 expression decreases rapidly during the late period of oil accumulation, which raises the possibility that other key genes might play important roles in triacylglycerol synthesis. Construction of a tung kernel cDNA library and related biochemical analyses was carried out. Notably, F-BOX genes and oleosin-coding genes responsible for oil body structure were the most abundant ESTs in our cDNA library. All the results indicate that F-BOX and oleosin might be actively involved in rung lipid metabolism and serve as additional candidate genes for genetic engineering of the tung tree. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.

引用

页码：684 / 686

页数：3

共 10 条

[1] BEHAVIOR OF DIGLYCERIDES AND CONJUGATED FATTY-ACID TRIGLYCERIDES IN REVERSE-PHASE CHROMATOGRAPHY [J].

CHANG, MK ;

CONKERTON, EJ ;

CHAPITAL, D ;

WAN, PJ .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1994, 71 (10) :1173-1175

[2]

Chen YC, 2008, AFR J BIOTECHNOL, V7, P4743

[3] Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content [J].

Clemente, Tom E. ;

Cahoon, Edgar B. .

PLANT PHYSIOLOGY, 2009, 151 (03) :1030-1040

[4] Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity [J].

Dyer, JM ;

Chapital, DC ;

Kuan, JCW ;

Mullen, RT ;

Turner, C ;

McKeon, TA ;

Pepperman, AB .

PLANT PHYSIOLOGY, 2002, 130 (04) :2027-2038

[5] F-box proteins take center stage [J].

Eckardt, NA .

PLANT CELL, 2004, 16 (03) :558-561

[6] Production and characterization of biodiesel from tung oil [J].

Park, Ji-Yeon ;

Kim, Deog-Keun ;

Wang, Zhong-Ming ;

Lu, Pengmei ;

Park, Soon-Chul ;

Lee, Jin-Suk .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2008, 148 (1-3) :109-117

[7] Palm oil and palm kernel oil as raw materials for basic oleochemicals and biodiesel [J].

Rupilius, Wolfgang ;

Ahmad, Salmiah .

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 2007, 109 (04) :433-439

[8] Properties of Tung oil biodiesel and its blends with 0# diesel [J].

Shang, Qiong ;

Jiang, Wei ;

Lu, Houfang ;

Liang, Bin .

BIORESOURCE TECHNOLOGY, 2010, 101 (02) :826-828

[9] Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum [J].

Shockey, Jay M. ;

Gidda, Satinder K. ;

Chapital, Dorselyn C. ;

Kuan, Jui-Chang ;

Dhanoa, Preetinder K. ;

Bland, John M. ;

Rothstein, Steven J. ;

Mullen, Robert T. ;

Dyer, John M. .

PLANT CELL, 2006, 18 (09) :2294-2313

[10] The accumulation of oleosins determines the size of seed oilbodies in Arabidopsis [J].

Siloto, Rodrigo M. P. ;

Findlay, Kim ;

Lopez-Villalobos, Arturo ;

Yeung, Edward C. ;

Nykiforuk, Cory L. ;

Moloney, Maurice M. .

PLANT CELL, 2006, 18 (08) :1961-1974

← 1 →