Enzymatic synthesis of fatty acid methyl esters from lard with immobilized Candida sp 99-125

被引：126

作者：

Lu, Jike ^{[1
]}

Nie, Kaili ^{[1
]}

Xie, Feng ^{[1
]}

Wang, Fang ^{[1
]}

Tan, Tianwei ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Bioproc Key Lab, Beijing 100029, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2007年 / 42卷 / 09期

基金：

中国国家自然科学基金;

关键词：

biodiesel; immobilized lipase; transesterification; lard; methanolysis; optimization; BIODIESEL FUEL PRODUCTION; ALKYL ESTERS; SOYBEAN OIL; LIPASE; OPTIMIZATION; GREASE;

D O I：

10.1016/j.procbio.2007.06.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, immobilized lipase catalyzed biodiesel production from lard was studied. Using Candida sp. 99-125, the effect of temperature, water content, enzyme amount, solvent and three-step methanolysis were investigated. The optimal conditions for processing 1 g of lard were: 0.2 g immobilized lipase, 8 ml n-hexane as solvent, 20% water based on the fat weight, temperature 40 degrees C, and three-step addition of methanol. As a result, the fatty acid methyl esters (FAMEs) yield was 87.4%. The lipase was proved to be stable when used repeatedly for 180 h. (c) 2007 Published by Elsevier Ltd.

引用

页码：1367 / 1370

页数：4

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