Effect of chlorhexidine application on long-term shear bond strength of resin cements to dentin

Purpose: The purpose of this study was to investigate the effect of chlorhexidine [CH] on dentin bond strength of three resin cements after 1 year of water storage. Methods: A flat middle dentin surface was prepared on 120 extracted premolars. The teeth were randomly divided into 6 groups of 20 specimens each according to the resin cement used: Panavia F2.0, Variolink II, and RelyX Unicem, with or without CH application. After cementation of an indirect composite rod [Z250], one subgroup [n = 10] was tested after 24 h in water at 37 degrees C and the other subgroup [n = 10] was tested after 1 year storage in water plus thermocycling. A shear bond strength [SBS] test was performed. The data [in MPa] were analyzed with ANOVA and Tukey tests [P < 0.05]. Results: Three-way ANOVA [resin cement, CH and time] indicated that Variolink II had the highest strength [16.65 +/- 3.60] and RelyX Unicem had the lowest strength [9.30 +/- 4.07]. Chlorhexidine application increased SBS [13.31 +/- 4.61] compared to samples without CH [12.16 +/- 5.04] [P = 0.04]. Initial SBS [15.63 +/- 4.37] was significantly higher than after 1 year of storage [9.85 +/- 3.36] [P < 0.001]. Separate two-way ANOVA for 24-h and 1-year data showed that cement had a significant effect but CH and its interaction had no significant effect at 24 h, whereas at 1 year the two factors and their interaction differed significantly [P <= 0.001]. Conclusions: Chlorhexidine 2% can diminish the loss of bonding effectiveness over time associated to etch-and-rinse and self-etch cements, although it appears not have any effect on self-adhesive cement. (C) 2010 Japan Prosthodontic Society. Published by Elsevier Ireland. All rights reserved.