Fluid dynamics during bleb formation in migrating cells in vivo

被引：11

作者：

Goudarzi, Mohammad ^{[1
,4
]}

Boquet-Pujadas, Aleix ^{[2
,3
]}

Olivo-Marin, Jean-Christophe ^{[2
,3
]}

Raz, Erez ^{[1
]}

机构：

[1] ZMBE, Inst Cell Biol, Munster, Germany

[2] Inst Pasteur, Bioimage Anal Unit, Paris, France

[3] CNRS, UMR3691, Paris, France

[4] Inst Sci & Technol Austria IST, Campus 1, Klosterneuburg, Austria

来源：

PLOS ONE | 2019年 / 14卷 / 02期

基金：

欧盟地平线“2020”; 欧洲研究理事会;

关键词：

MEMBRANE; MOTILITY; PRESSURE; BEHAVIOR; MODES;

D O I：

10.1371/journal.pone.0212699

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.

引用

页数：11

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