Performance of invertase immobilized on glass-ceramic supports in batch bioreactor

被引：10

作者：

Albertini, A. V. P. ^{[1
]}

Cadena, P. G. ^{[1
]}

Silva, J. L. ^{[2
]}

Nascimento, G. A. ^{[1
]}

Reis, A. L. S. ^{[1
]}

Freire, V. N. ^{[3
]}

Santos, R. P. ^{[5
]}

Martins, J. L. ^{[7
]}

Cavada, B. S. ^{[4
]}

Rolim Neto, P. J. ^{[2
]}

Pimentel, M. C. B. ^{[1
]}

Martinez, C. R. ^{[6
]}

Porto, A. L. F. ^{[1
]}

Lima Filho, J. L. ^{[1
]}

机构：

[1] Univ Fed Pernambuco UFPE, LIKA, BR-50780901 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Dept Ciencias Farmaceut, Lab Tecnol Medicamentos, BR-50740521 Recife, PE, Brazil

[3] Univ Fed Ceara, Dept Fis, BR-60455900 Fortaleza, Ceara, Brazil

[4] Univ Fed Ceara, Lab Mol Biol Ativas BioMol Lab, BR-60440970 Fortaleza, Ceara, Brazil

[5] Univ Fed Ceara, Lab Nanotecnol & Bioestat Sobral NanoBioS Lab Eng, BR-62042280 Sobral, Ceara, Brazil

[6] Univ Fed Paraiba, Dept Quim, BR-58059900 Joao Pessoa, Paraiba, Brazil

[7] Univ Fed Pelotas, Inst Quim & Geociencias, BR-96160000 Pelotas, RS, Brazil

来源：

CHEMICAL ENGINEERING JOURNAL | 2012年 / 187卷

关键词：

Coal fly ashes; Glass-ceramic supports; Invertase; Immobilization; Performance; COAL FLY-ASH; POLYVINYL PYRROLIDONE; HYDROLYSIS; SUCROSE; GLUCOAMYLASE; VALORIZATION; ADSORPTION; CARRIERS; WASTES; MATRIX;

D O I：

10.1016/j.cej.2012.01.091

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Invertase was covalently immobilized on glass-ceramic support (G(S) obtained from coal fly ashes (CFA) with polyvinyl pyrrolidone (PVP) and magnesium stearate added as temporary additive. The GCS samples containing Pb(CH3COO)(2), (GCSPb) or ZnSO4 (GCSZn) were obtained in several sintering temperatures (1000-1200 degrees C) and times (1-3 h). The apparent values of porosity, water absorption, density and uniaxial compressive strength were studied by factorial design and morphological differences by scanning electron microscope. The best covalent immobilized invertase derivative was on GCSZn (1200 degrees C/1 h) with 4398 +/- 59.75 U/g GCS. However, 95.83% of immobilized invertase activity was obtained using an economical protocol with concentration of 0.636 mM 3-aminopropyltriethoxy silane (3-APTES) and 0.576 mM glutaraldehycle. The GCSZn-invertase derivative retained 100% of initial activity after nine reuses (5476.19 +/- 155.49 U/g GCSZn). The CFA showed to be a promising biotechnological alternative as immobilization support for production of invert sugar and may be for other industrial enzymes. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：341 / 350

页数：10

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