Intravaginal Delivery of Syngonanthus nitens (Bong.) Ruhland Fraction Based on a Nanoemulsion System Applied to Vulvovaginal Candidiasis Treatment

被引：27

作者：

dos Santos Ramos, Matheus Aparecido ^{[1
]}

da Silva, Patricia Bento ^{[2
]}

de Toledo, Luciani Gaspar ^{[1
]}

Oda, Fernando Bombarda ^{[3
]}

da Silva, Isabel Cristiane ^{[1
]}

dos Santos, Lourdes Campaner ^{[4
]}

dos Santos, Andre Gonzaga ^{[3
]}

Gottardo de Almeida, Margarete Teresa ^{[5
]}

Pavan, Fernando Rogerio ^{[1
]}

Chorilli, Marius ^{[2
]}

Bauab, This Maria ^{[1
]}

机构：

[1] Sao Paulo State Univ UNESP, Dept Biol Sci, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP, Brazil

[2] Sao Paulo State Univ UNESP, Dept Drug & Med, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP, Brazil

[3] Sao Paulo State Univ UNESP, Dept Nat Prod & Toxicol, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP, Brazil

[4] Sao Paulo State Univ UNESP, Dept Organ Chem, Chem Inst Campus Araraquara, BR-14800060 Sao Paulo, SP, Brazil

[5] Med Sch Sao Jose Rio Preto FAMERP, Dept Infect Dis, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2019年 / 15卷 / 05期

基金：

巴西圣保罗研究基金会;

关键词：

Syngonanthus nitens; Luteolin; Vulvovaginal Candidiasis; Nanoemulsion; Therapeutic Activity; PRECURSOR MUCOADHESIVE SYSTEM; NANOSTRUCTURED LIPID SYSTEM; ANTIMICROBIAL ACTIVITY; IN-VITRO; CHEMOPREVENTIVE ACTIVITIES; ANTIBACTERIAL ACTIVITY; ALBICANS-ACTIVITY; STRATEGY; EXTRACT; IMPROVE;

D O I：

10.1166/jbn.2019.2750

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

In this study, was evaluated the chemical composition of a fraction from Syngonanthus nitens extract and its antimicrobial potential unloaded (Fr3) and loaded (F9Fr3) into a nanoemulsion (F9) composed of cholesterol as the oil phase (10%), polyoxyethylene 20-cetyl ether and soy phosphatidylcholine (2:1) as surfactant (20%), and a solution of phosphate buffer (pH 7.4) plus chitosan polymer dispersion (0.25%) as the aqueous phase (70%) to use for VVC treatment. Phytochemical procedures showed that Fr3 is rich in luteolin, which is responsible for the antimicrobial activity. F9 development showed satisfactory parameters for use in the vulvovaginal candidiasis (VVC) treatment, as F9 demonstrated pseudoplastic, elastic behavior, and adhesive properties on vaginal mucosa. In addition, we observed improvement in antimicrobial potential of Fr3 on planktonic and biofilms after incorporation in F9. Fr3 and F9Fr3 showed satisfactory parameters related to toxic profiles in cell lines and in a model of acute toxicity by Artemia salina. The in vivo VVC assay showed that F9Fr3 was more active than unloaded Fr3 in VVC treatment. In conclusion, this work showed that use of a fraction rich in luteolin can be a used as an antimicrobial for treatment of vaginal infections and that use of nanostructured lipid systems was an important factor in the biological efficacy of Fr3, especially in treatment of acute VVC.

引用

页码：1072 / 1089

页数：18

共 59 条

[1] Composition and antimicrobial activity of the essential oils of two Origanum species [J].

Aligiannis, N ;

Kalpoutzakis, E ;

Mitaku, S ;

Chinou, IB .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (09) :4168-4170

[2] Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp. [J].

Bonifacio, Bruna Vidal ;

dos Santos Ramos, Matheus Aparecido ;

da Silva, Patricia Bento ;

Silveira Negri, Kamila Maria ;

Lopes, Erica de Oliveira ;

de Souza, Leonardo Perez ;

Vilegas, Wagner ;

Pavan, Fernando Rogerio ;

Chorilli, Marlus ;

Bauab, Tais Maria .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2015, 10 :5081-5092

[3] Nanotechnology-based drug delivery systems and herbal medicines: a review [J].

Bonifacio, Bruna Vidal ;

da Silva, Patricia Bento ;

dos Santos Ramos, Matheus Aparecido ;

Silveira Negri, Kamila Maria ;

Bauab, Tais Maria ;

Chorilli, Marlus .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2014, 9 :1-15

[4] Development of positively charged colloidal drug carriers: Chitosan coated polyester nanocapsules and submicron-emulsions [J].

Calvo, P ;

RemunanLopez, C ;

VilaJato, JL ;

Alonso, MJ .

COLLOID AND POLYMER SCIENCE, 1997, 275 (01) :46-53

[5] Antifungal activity of the honey flavonoid extract against Candida albicans [J].

Cancliracci, Manila ;

Citterio, Barbara ;

Piatti, Elena .

FOOD CHEMISTRY, 2012, 131 (02) :493-499

[6] Identification of chicoric acid as a hypoglycemic agent from Ocimum gratissimum leaf extract in a biomonitoring in vivo study [J].

Casanova, Livia Marques ;

da Silva, Daniel ;

Sola-Penna, Mauro ;

de Magalhaes Camargo, Luiza Maria ;

Celestrini, Deborah de Moura ;

Tinoco, Luzineide Wanderley ;

Costa, Sonia Soares .

FITOTERAPIA, 2014, 93 :132-141

[7]

CLSI, 2008, M27A3 CLSI

[8]

CLSI, 2006, M7A6 CLSI

[9] Natural Products as a Vital Source for the Discovery of Cancer Chemotherapeutic and Chemopreventive Agents [J].

Cragg, Gordon M. ;

Pezzuto, John M. .

MEDICAL PRINCIPLES AND PRACTICE, 2016, 25 :41-59

[10] A Nanostructured Lipid System as a Strategy to Improve the in Vitro Antibacterial Activity of Copper(II) Complexes [J].

da Silva, Patricia B. ;

Bonifacio, Bruna V. ;

Frem, Regina C. G. ;

Godoy Netto, Adelino V. ;

Mauro, Antonio E. ;

da Costa Ferreira, Ana M. ;

Lopes, Erica de O. ;

Raddi, Maria S. G. ;

Bauab, Tais M. ;

Pavan, Fernando R. ;

Chorilli, Marlus .

MOLECULES, 2015, 20 (12) :22534-22545

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