Macromolecular properties of Diocleinae lectins

被引:0
|
作者
Ramos, MV
Beltramini, LM
Moreira, RA
机构
[1] Univ Fed Ceara, Dept Biol, BR-60451970 Fortaleza, Ceara, Brazil
[2] Univ Sao Paulo, Lab Biofis, Inst Fis & Quim Sao Carlos, BR-05508 Sao Paulo, Brazil
来源
PROTEIN AND PEPTIDE LETTERS | 2001年 / 8卷 / 05期
关键词
D O I
10.2174/0929866013409193
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Structural properties of six closely related lectins isolated from plants belonging from the legume group Diocleinae were studied in solution. Affinity and gel filtration chromatography of the lectins at different pH conditions demonstrated that the lectins diverge in affinity for a specific matrix mainly at the pH range 4.0 up to 6.0 and that at neutral pH they tend to form dimers. Analysis of circular dichroism and fluorescence emission spectra stressed the differences of the lectin from Cratylia floribunda seeds from that of Canavalia brasiliensis, C. ensisformis, C. maritima, Diolcea grandiflora and D. virgata. According to the results, these proteins constitute a excellent model to study fine protein structure/function relationships.
引用
收藏
页码:399 / 406
页数:8
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