A polydisperse linear random coil model for the quaternary structure of pig colonic mucin

被引：22

作者：

Jumel, K ^{[1
]}

Fogg, FJJ ^{[1
]}

Hutton, DA ^{[1
]}

Pearson, JP ^{[1
]}

Allen, A ^{[1
]}

Harding, SE ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE, SCH MED, DEPT PHYSIOL SCI, NEWCASTLE UPON TYNE NE2 4HH, TYNE & WEAR, ENGLAND

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 1997年 / 25卷 / 5-6期

关键词：

mucin conformation; molecular weight distribution; CERVICAL-MUCUS GLYCOPROTEINS; GEL;

D O I：

10.1007/s002490050063

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The distribution of molecular weights for polymeric colonic mucus glycoprotein or ''mucin'' isolated and solubilised in the presence of protease inhibitors from pig colons is shown to be considerably greater than its ''subunit'' (thiol reduction product) and papain digested forms using the technique of size-exclusion chromatography coupled to multi-angle laser light scattering, and confirmed by sedimentation equilibrium measurements. The conformation of this mucin is probed by examining the molecular weight - intrinsic viscosity relationship in terms of the Mark-Houwink-Kuhn-Sakurada analysis for its polymeric (or ''whole''), reduced and papain-digested forms: an exponent ''alpha'' of (1.1+/-0.1) is obtained indicating a linear random coil conformation consistent with other mucins. Size-exclusion chromatography coupled to multi-angle laser light scattering is shown to provide a relatively simple complementary technique to sedimentation equilibrium for the molecular weight distribution analysis of polydisperse materials.

引用

页码：477 / 480

页数：4

共 22 条

[1]

Allen A., 1981, PHYSL GASTROINTESTIN, P617

[2] EXPRESSION OF HUMAN MUCIN GENES IN RESPIRATORY, DIGESTIVE, AND REPRODUCTIVE TRACTS ASCERTAINED BY IN-SITU HYBRIDIZATION [J].