Unprecedented Ultra-High-Resolution Hydroxy Group 1H NMR Spectroscopic Analysis of Plant Extracts

被引:40
作者
Charisiadis, Pantelis [1 ]
Primikyri, Alexandra [1 ]
Exarchou, Vassiliki [2 ]
Tzakos, Andreas [1 ]
Gerothanassis, Ioannis P. [1 ]
机构
[1] Univ Ioannina, Dept Chem, Sect Organ Chem & Biochem, GR-45110 Ioannina, Greece
[2] Univ Ioannina, NMR Ctr, GR-45110 Ioannina, Greece
来源
JOURNAL OF NATURAL PRODUCTS | 2011年 / 74卷 / 11期
关键词
MAGNETIC-RESONANCE SPECTROSCOPY; CHARGE-TRANSFER COMPLEXES; ACIDS; QUANTIFICATION; MIXTURES; EXCHANGE; CANCER; MEDIA;
D O I
10.1021/np200329a
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A general method is demonstrated for obtaining ultra-high resolution in the phenolic hydroxy group H-1 NMR spectroscopic region, in DMSO-d(6) solution, with the addition of picric acid. Line-width reduction by a factor of over 100 was observed, which resulted in line-widths ranging from 1.6 to 0.6 Hz. This unprecedented resolution, in combination with the shielding sensitivity of the hydroxy group absorptions to substituent effects at least up to 11 bonds distant and the application of 2D H-1-C-13 HMBC techniques, allows the unequivocal structure analysis of natural products with phenolic hydroxy groups in complex plant extracts.
引用
收藏
页码:2462 / 2466
页数:5
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