CTGF/CCN2 has a chemoattractive function but a weak adhesive property to embryonic carcinoma cells

被引：4

作者：

Aguiar, Diego P. ^{[1
]}

Pontes, Bruno ^{[2
]}

Mendes, Fabio A. ^{[1
]}

Andrade, Leonardo R. ^{[3
]}

Viana, Nathan B. ^{[2
,4
]}

Abreu, Jose G. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Lab Embriol Vertebrados, BR-21941902 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, LPO COPEA, BR-21941902 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Lab Biomineralizacao, BR-21941902 Rio De Janeiro, Brazil

[4] Univ Fed Rio de Janeiro, Inst Fis, BR-21941902 Rio De Janeiro, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 413卷 / 04期

关键词：

Adhesion; CCN2; Chemoattraction; Migration; Optical tweezers; P19; cells; TISSUE GROWTH-FACTOR; OPTICAL TWEEZERS; STEM-CELLS; MIGRATION; DIFFERENTIATION; PROLIFERATION; ANGIOGENESIS; CYTOSKELETON; EXPRESSION; CCN2/CTGF;

D O I：

10.1016/j.bbrc.2011.09.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Connective tissue growth factor (CTGF/CCN2) is a protein of the CCN family that modulates cell-ECM interactions in a variety of cell types. In this study, we investigated the chemotactic and adhesive properties of CCN2 protein in embryonic teratocarcinoma P19 cells. Initially. P19 cells were attracted to CCN2-coated agarose beads. In Boyden chamber experiments, CCN2-containing medium induced a threefold greater migration of P19 cells. CCN2 adhesion properties were studied by using optical tweezers. The specific adhesion times of P19 cells to polystyrene beads coated with laminin, fibronectin, CCN2 and bovine serum albumin were 1.8 +/- 0.5s, 2.7 +/- 0.4s, 10 +/- 2s and 13 +/- 2s, respectively, revealing an unexpectedly low adhesive capacity of CCN2 protein for P19 cells. In conclusion, our findings support the chemoattractive role of CCN2 for P19 cells, but not its adhesive role when compared to laminin or fibronectin. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：582 / 587

页数：6

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