Purification, chemical analysis and inhibitory effects on galectin-3 of enzymatic pH-modified citrus pectin

被引：7

作者：

Zhang, Tao ^{[1
,2
]}

Sun, Guoqing ^{[3
]}

Shuai, Ming ^{[2
]}

Ye, Jingyu ^{[2
]}

Huang, Jian ^{[1
,2
]}

Yao, Xiaodong ^{[3
]}

Sun, Chengxin ^{[3
]}

Min, Xun ^{[1
,2
]}

机构：

[1] Zunyi Med Univ, Dept Lab Med, Affiliated Hosp, Zunyi 563003, Guizhou, Peoples R China

[2] Zunyi Med Univ, Sch Lab Med, Zunyi 563006, Guizhou, Peoples R China

[3] Zunyi Med Univ, Sch Pharm, Zunyi 563006, Guizhou, Peoples R China

来源：

FOOD CHEMISTRY-X | 2021年 / 12卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Modified citrus pectin; Polygalacturonase hydrolysis; Purification; Chemical analysis; Galectin-3; inhibitor; ACIDIC POLYSACCHARIDE; RHAMNOGALACTURONAN-II; COMPLEX;

D O I：

10.1016/j.fochx.2021.100169

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Modified citrus pectin (MCP), a commercially available dietary supplement prepared from citrus pectin, contains several different polysaccharide domains, but its primary chemical structure and the binding epitopes that antagonize galectin-3 function remain unclear. In this study, five fractions were isolated from MCP after endopolygalacturonase degradation (EMCP) and a combination of DEAE-cellulose and Sepharose CL-6B or Sephadex G-75 chromatography. Their primary structures, abilities to inhibit galectin-3-mediated hemagglutination, and antiproliferation activities on MCF-7 and A549 cell lines were studied. Results showed that EMCP-3p, one of the five fractions, was composed of Glc (89.8%), Gal (3.8%), Ara (3.1%), GalA (1.1%), Man (0.9%), and Rha (1.3%) with an average molecular weight of 88.4 KDa, which had the most substantial degree of galectin-3 inhibition with an MIC of 31.25 mu g/mL, and it exhibited remarkable cytotoxicity against MCF-7 (36.7%) and A549 (57.4%) cell lines. These results provide new insight into the structure-function relationships of EMCP-derived polysaccharides.

引用

页数：8