Hyaluronic acid biomaterial for human tissue-engineered skin substitutes: Preclinical comparative in vivo study of wound healing

被引：38

作者：

Sierra-Sanchez, A. ^{[1
,2
]}

Fernandez-Gonzalez, A. ^{[1
,2
]}

Lizana-Moreno, A. ^{[1
,2
]}

Espinosa-Ibanez, O. ^{[1
,2
]}

Martinez-Lopez, A. ^{[2
,3
]}

Guerrero-Calvo, J. ^{[1
,2
]}

Fernandez-Porcel, N. ^{[1
,2
]}

Ruiz-Garcia, A. ^{[1
,2
]}

Ordonez-Luque, A. ^{[1
,2
]}

Carriel, V ^{[2
,4
,5
]}

Arias-Santiago, S. ^{[1
,2
,3
,6
]}

机构：

[1] Virgen de las Nieves Univ Hosp, Cell Prod & Tissue Engn Unit, Andalusian Network Design & Translat Adv Therapie, Granada, Spain

[2] Biosanit Inst Granada Ibs GRANADA, Granada, Spain

[3] Virgen de las Nieves Univ Hosp, Dermatol Dept, Granada, Spain

[4] Univ Granada, Fac Med, Dept Histol, Granada, Spain

[5] Univ Granada, Fac Med, Tissue Engn Grp, Granada, Spain

[6] Univ Granada, Fac Med, Dermatol Dept, Granada, Spain

来源：

JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY | 2020年 / 34卷 / 10期

关键词：

HYDROGELS; SCAFFOLD; PATIENT; FILLERS; SCARS;

D O I：

10.1111/jdv.16342

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background There is not an ideal biomaterial for tissue-engineered skin substitutes (TESSs), and most of the studies or existing therapies use xenogeneic origin natural biomaterials or biosynthetic scaffolds. Objective To analyse clinical, histological integration and homeostasis parameters of a human TESS manufactured with fibrin-hyaluronic acid biomaterial (HA-Skin), grafted in immunodeficient mice for 8 weeks, and compared with the gold standard treatment (Autograft), a human TESS manufactured with fibrin-agarose biomaterial (AG-Skin) and secondary wound healing dressings. Methods Human TESSs and autografts were implanted into BALB/c mice after surgical excision. Secondary wound healing approach was achieved with biosynthetic collagen wound dressing (Biobrane(R)) and fibrin-hyaluronic acid or fibrin-agarose biomaterial without cells (Total N = 44). Clinical integration and homeostasis parameters were evaluated every two weeks for two months. Histological and immunohistochemical analyses were performed four and eight weeks after grafting. Results HA-Skin, AG-Skin and Autograft groups showed a proper clinical integration and epithelization eight weeks later. Scar evaluation revealed better results for Autograft and HA-Skin. Homeostasis analysis indicated similar values of transepidermal water loss and elasticity between HA-Skin (6.42 +/- 0.75 g/h/m(2), 0.42 +/- 0.08 mu m), Autograft (6.91 +/- 1.28 g/h/m(2), 0.40 +/- 0.08 mu m) and healthy mouse skin (6.40 +/- 0.43 g/h/m(2), 0.35 +/- 0.03 mu m). Histological results showed that human TESSs and autografts presented better skin structuration and higher expression of cytokeratins. Conclusions This study suggests that human TESS based on fibrin-hyaluronic acid biomaterial could be suitable for clinical application in the treatment of several dermatological pathologies (wound healing).

引用

页码：2414 / 2427

页数：14

共 43 条

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