Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity

被引：10

作者：

Usui, Kenji ^{[1
,2
,3
,4
]}

Kakiyama, Takashi ^{[1
]}

Tomizaki, Kin-ya ^{[1
,5
,6
]}

Mie, Masayasu ^{[2
]}

Kobatake, Eiry ^{[2
]}

Mihara, Hisakazu ^{[1
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Bioengn, Yokohama, Kanagawa 2268501, Japan

[2] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Yokohama, Kanagawa 2268501, Japan

[3] Konan Univ, FIRST Fac Frontiers Innovat Res Sci & Technol, Kobe, Hyogo 6500047, Japan

[4] Konan Univ, FIBER, Kobe, Hyogo 6500047, Japan

[5] Ryukoku Univ, Innovat Mat & Proc Res Ctr, Otsu, Shiga 5202194, Japan

[6] Ryukoku Univ, Dept Chem Mat, Otsu, Shiga 5202194, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2011年 / 21卷 / 21期

基金：

日本科学技术振兴机构;

关键词：

Ligand design; Peptide library; Cell-based screening; Cytotoxicity; PROTEIN; ARRAY; MICROARRAYS; MEMBRANE;

D O I：

10.1016/j.bmcl.2011.09.002

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We conducted cell-based cytotoxicity screening of a 101-membered alpha-helical peptide library using cell fingerprints (CFPs). The CFP data suggested that there is a relationship between cytotoxicity and peptide characteristics, such as hydrophobicity, charge, and amino acid composition. In spite of the small size of the library used in this study, several peptides demonstrated cell-specific toxicity. The strategy of combining a designed peptide library with CFP thus shows real promise for peptide-based screening with cells. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：6281 / 6284

页数：4

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