Effect of Traumeel S on Cytokine Profile in a Cecal Ligation and Puncture (CLP) Sepsis Model in Rats

Background: Sepsis results in significant morbidity and mortality, with current treatment options limited with respect to efficacy as well as safety. The complex homeopathic remedy Traumeel S has been shown to have both anti-inflammatory and immunostimulatory effects in the in vitro setting. Objectives: The objective was to explore the effects of Traumeel S in an in vivo setting, using a cecal ligation and puncture (CLP) sepsis model in rats, evaluating the effects of the medication on cytokine activity. Design: Sepsis was induced in 30 rats using accepted CLP methodology. Following the procedure, rats were randomly allocated to receive an intraperitoneal injection of either Traumeel S (n = 15) or normal saline (n = 15). At 6 hours post-CLP, serum cytokines (interleukin [IL]-1 beta, tumor necrosis factor-a, IL-6, and IL-10) were evaluated. Results: IL-1 beta levels were significantly higher in the treatment group (p = 0.03) with no significant differences found between the groups with respect to the other cytokines tested. Conclusions: In contrast to in vitro studies, Traumeel significantly increased IL-1 beta levels in an in vivo model, without influencing other cytokines. IL-1 beta is a proinflammatory cytokine that has been shown to have a protective effect in the CLP rat model. Further research is warranted to examine this finding, as well as its clinical implications.