Cytotoxicity of marine-derived fungi collected from Kepulauan Seribu Marine National Park

Marine-derived fungi are of great interest as new promising sources of biologically active products due to its diversity in chemical structures and biological activities. The objective of this study was aimed to determine the cytotoxicity of marine sponge-derived fungi that collected from Kepulauan Seribu Marine National Park. The fungi were fermented in a static liquid culture of malt extract broth medium (containing 0.3% malt extract, 0.3% yeast extract, 0.5% peptone and seawater) for 3 weeks at 27-29 degrees C. Bioactive secondary metabolites of fungi were extracted using ethyl acetate. Cytotoxicity of the extract was performed by using MTT assay. A total of 17 isolates were tested against T47D cell at a concentration of 30 mu g/mL, among them, isolate of MFP270 exhibited the strongest cytotoxicity. Further analysis showed that mycelium extract of MFP270 had stronger cytotoxicity (IC50 = 28.3 mu g/mL) than broth extract (IC50 = 645 mu g/mL). The MFP270 marine fungal was identified as Aspergillus sp. base on its morphology features.