CRISPR-Powered Microfluidics in Diagnostics: A Review of Main Applications

被引：15

作者：

Azimzadeh, Mostafa ^{[1
,2
,3
]}

Mousazadeh, Marziyeh ^{[4
]}

Jahangiri-Manesh, Atieh ^{[4
]}

Khashayar, Pouria ^{[5
]}

Khashayar, Patricia ^{[6
,7
]}

机构：

[1] Shahid Sadoughi Univ Med Sci, Sch Med, Dept Med Biotechnol, Yazd 89165887, Iran

[2] Shahid Sadoughi Univ Med Sci, Yazd Reprod Sci Inst, Med Nanotechnol & Tissue Engn Res Ctr, Yazd 89195999, Iran

[3] Shahid Sadoughi Univ Med Sci, Yazd Reprod Sci Inst, Stem Cell Biol Res Ctr, Yazd 89195999, Iran

[4] Tarbiat Modares Univ, Fac Biol Sci, Dept Nanobiotechnol, Tehran 14115111, Iran

[5] Univ Glasgow, Inst Cardiovasc & Med Sci, Glasgow G12 8QQ, Lanark, Scotland

[6] Imec, Ctr Microsyst Technol, B-9050 Ghent, Belgium

[7] Univ Ghent, B-9050 Ghent, Belgium

来源：

CHEMOSENSORS | 2022年 / 10卷 / 01期

关键词：

microfluidics; lab-on-chip; CRISPR; biosensor; medical detection; NUCLEIC-ACID DETECTION; ASSAY; DELIVERY; CAS12A; FUTURE;

D O I：

10.3390/chemosensors10010003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the past few years, the CRISPR (clustered regularly interspaced short palindromic repeats) applications in medicine and molecular biology have broadened. CRISPR has also been integrated with microfluidic-based biosensors to enhance the sensitivity and selectivity of medical diagnosis due to its great potentials. The CRISPR-powered microfluidics can help quantify DNAs and RNAs for different diseases such as cancer, and viral or bacterial diseases among others. Here in this review, we discussed the main applications of such tools along with their advantages and limitations.

引用

页数：20

共 50 条

[41] Origami Microfluidics: A Review of Research Progress and Biomedical Applications
Kaixin Sun
Yiqiang Fan
Michael Hebda
Yajun Zhang
Biomedical Materials & Devices, 2023, 1 (1): : 388 - 401
[42] A Review on the Role of Microflow Parameter Measurements for Microfluidics Applications
Kotreshappa, Sreedevi Lingadahalli
Nayak, Chempi Gurudas
Venkata, Santhosh Krishnan
SYSTEMS, 2023, 11 (03):
[43] A Review of Cyclic Olefin Copolymer Applications in Microfluidics and Microdevices
Agha, Abdulrahman
Waheed, Waqas
Alamoodi, Nahla
Mathew, Bobby
Alnaimat, Fadi
Abu-Nada, Eiyad
Abderrahmane, Aissa
Alazzam, Anas
MACROMOLECULAR MATERIALS AND ENGINEERING, 2022, 307 (08)
[44] Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell-Mediated Biomedical Applications
Zheng, Fangqiao
Tian, Ruizhi
Lu, Hongxu
Liang, Xiao
Shafiq, Muhammad
Uchida, Satoshi
Chen, Hangrong
Ma, Ming
SMALL, 2024, 20 (42)
[45] Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets
Kadam, Ulhas Sopanrao
Cho, Yuhan
Park, Tae Yoon
Hong, Jong Chan
APPLIED BIOLOGICAL CHEMISTRY, 2023, 66 (01)
[46] A CRISPR/Cas13a-powered catalytic electrochemical biosensor for successive and highly sensitive RNA diagnostics
Sheng, Yan
Zhang, Tenghua
Zhang, Shihong
Johnston, Midori
Zheng, Xiaohe
Shan, Yuanyue
Liu, Tong
Huang, Zena
Qian, Feiyang
Xie, Zihui
Ai, Yiru
Zhong, Hankang
Kuang, Tairong
Dincer, Can
Urban, Gerald Anton
Hu, Jiaming
BIOSENSORS & BIOELECTRONICS, 2021, 178
[47] Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets
Ulhas Sopanrao Kadam
Yuhan Cho
Tae Yoon Park
Jong Chan Hong
Applied Biological Chemistry, 66
[48] Magnetic nanoparticles fabricated/integrated with microfluidics for biological applications: A review
Mahtab Ghasemi Toudeshkchouei
Hassan Abdoos
Biomedical Microdevices, 2024, 26
[49] Magnetic nanoparticles fabricated/integrated with microfluidics for biological applications: A review
Toudeshkchouei, Mahtab Ghasemi
Abdoos, Hassan
BIOMEDICAL MICRODEVICES, 2024, 26 (01)
[50] Microfluidics-to-mass spectrometry: A review of coupling methods and applications
Wang, Xue
Yi, Lian
Mukhitov, Nikita
Schrell, Adrian M.
Dhumpa, Raghuram
Roper, Michael G.
JOURNAL OF CHROMATOGRAPHY A, 2015, 1382 : 98 - 116

← 1 2 3 4 5 →