CRISPR-Powered Microfluidics in Diagnostics: A Review of Main Applications

被引：15
作者：
Azimzadeh, Mostafa [1 ,2 ,3 ]
Mousazadeh, Marziyeh [4 ]
Jahangiri-Manesh, Atieh [4 ]
Khashayar, Pouria [5 ]
Khashayar, Patricia [6 ,7 ]
机构：
[1] Shahid Sadoughi Univ Med Sci, Sch Med, Dept Med Biotechnol, Yazd 89165887, Iran
[2] Shahid Sadoughi Univ Med Sci, Yazd Reprod Sci Inst, Med Nanotechnol & Tissue Engn Res Ctr, Yazd 89195999, Iran
[3] Shahid Sadoughi Univ Med Sci, Yazd Reprod Sci Inst, Stem Cell Biol Res Ctr, Yazd 89195999, Iran
[4] Tarbiat Modares Univ, Fac Biol Sci, Dept Nanobiotechnol, Tehran 14115111, Iran
[5] Univ Glasgow, Inst Cardiovasc & Med Sci, Glasgow G12 8QQ, Lanark, Scotland
[6] Imec, Ctr Microsyst Technol, B-9050 Ghent, Belgium
[7] Univ Ghent, B-9050 Ghent, Belgium
来源：
CHEMOSENSORS | 2022年 / 10卷 / 01期
关键词：
microfluidics; lab-on-chip; CRISPR; biosensor; medical detection; NUCLEIC-ACID DETECTION; ASSAY; DELIVERY; CAS12A; FUTURE;
D O I：
10.3390/chemosensors10010003
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In the past few years, the CRISPR (clustered regularly interspaced short palindromic repeats) applications in medicine and molecular biology have broadened. CRISPR has also been integrated with microfluidic-based biosensors to enhance the sensitivity and selectivity of medical diagnosis due to its great potentials. The CRISPR-powered microfluidics can help quantify DNAs and RNAs for different diseases such as cancer, and viral or bacterial diseases among others. Here in this review, we discussed the main applications of such tools along with their advantages and limitations.
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页数：20
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