CRISPR-Powered Microfluidics in Diagnostics: A Review of Main Applications

被引：15
作者：
Azimzadeh, Mostafa [1 ,2 ,3 ]
Mousazadeh, Marziyeh [4 ]
Jahangiri-Manesh, Atieh [4 ]
Khashayar, Pouria [5 ]
Khashayar, Patricia [6 ,7 ]
机构：
[1] Shahid Sadoughi Univ Med Sci, Sch Med, Dept Med Biotechnol, Yazd 89165887, Iran
[2] Shahid Sadoughi Univ Med Sci, Yazd Reprod Sci Inst, Med Nanotechnol & Tissue Engn Res Ctr, Yazd 89195999, Iran
[3] Shahid Sadoughi Univ Med Sci, Yazd Reprod Sci Inst, Stem Cell Biol Res Ctr, Yazd 89195999, Iran
[4] Tarbiat Modares Univ, Fac Biol Sci, Dept Nanobiotechnol, Tehran 14115111, Iran
[5] Univ Glasgow, Inst Cardiovasc & Med Sci, Glasgow G12 8QQ, Lanark, Scotland
[6] Imec, Ctr Microsyst Technol, B-9050 Ghent, Belgium
[7] Univ Ghent, B-9050 Ghent, Belgium
来源：
CHEMOSENSORS | 2022年 / 10卷 / 01期
关键词：
microfluidics; lab-on-chip; CRISPR; biosensor; medical detection; NUCLEIC-ACID DETECTION; ASSAY; DELIVERY; CAS12A; FUTURE;
D O I：
10.3390/chemosensors10010003
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In the past few years, the CRISPR (clustered regularly interspaced short palindromic repeats) applications in medicine and molecular biology have broadened. CRISPR has also been integrated with microfluidic-based biosensors to enhance the sensitivity and selectivity of medical diagnosis due to its great potentials. The CRISPR-powered microfluidics can help quantify DNAs and RNAs for different diseases such as cancer, and viral or bacterial diseases among others. Here in this review, we discussed the main applications of such tools along with their advantages and limitations.
引用
收藏
页数：20
相关论文
共 58 条
  • [1] Massively multiplexed nucleic acid detection with Cas13
    Ackerman, Cheri M.
    Myhrvold, Cameron
    Thakku, Sri Gowtham
    Freije, Catherine A.
    Metsky, Hayden C.
    Yang, David K.
    Ye, Simon H.
    Boehm, Chloe K.
    Kosoko-Thoroddsen, Tinna-Solveig F.
    Kehe, Jared
    Nguyen, Tien G.
    Carter, Amber
    Kulesa, Anthony
    Barnes, John R.
    Dugan, Vivien G.
    Hung, Deborah T.
    Blainey, Paul C.
    Sabeti, Pardis C.
    [J]. NATURE, 2020, 582 (7811) : 277 - +
  • [2] The CRISPR tool kit for genome editing and beyond
    Adli, Mazhar
    [J]. NATURE COMMUNICATIONS, 2018, 9
  • [3] Prismatic Deflection of Live Tumor Cells and Cell Clusters
    Aldridge, Peter M.
    Mukhopadhyay, Monorina
    Ahmed, Sharif U.
    Zhou, Wendi
    Christinck, Elisa
    Makonnen, Rhema
    Sargent, Edward H.
    Kelley, Shana O.
    [J]. ACS NANO, 2018, 12 (12) : 12692 - 12700
  • [4] Microfluidic devices for continuous production of pDNA/cationic liposome complexes for gene delivery and vaccine therapy
    Balbino, Tiago Albertini
    Azzoni, Adriano Rodrigues
    de la Torre, Lucimara Gaziola
    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2013, 111 : 203 - 210
  • [5] Verification of CRISPR editing and finding transgenic inserts by Xdrop indirect sequence capture followed by short- and long-read sequencing
    Blondal, Thorarinn
    Gamba, Cristina
    Jagd, Lea Moller
    Su, Ling
    Demirov, Dimiter
    Guo, Shuang
    Johnston, Camille M.
    Riising, Eva M.
    Wu, Xiaolin
    Mikkelsen, Marie J.
    Szabova, Ludmila
    Mouritzen, Peter
    [J]. METHODS, 2021, 191 : 68 - 77
  • [6] Development and validation of a multiplex qPCR assay for detection and relative quantification of HPV16 and HPV18 E6 and E7 oncogenes
    Bordigoni, Alexia
    Motte, Anne
    Tissot-Dupont, Herve
    Colson, Philippe
    Desnues, Christelle
    [J]. SCIENTIFIC REPORTS, 2021, 11 (01)
  • [7] Innovations in CRISPR technology
    Brooks, Alexandra K.
    Gaj, Thomas
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2018, 52 : 95 - 101
  • [8] CRISPR-powered electrochemical microfluidic multiplexed biosensor for target amplification-free miRNA diagnostics
    Bruch, Richard
    Johnston, Midori
    Kling, Andre
    Mattmueller, Thorsten
    Baaske, Julia
    Partel, Stefan
    Madlener, Sibylle
    Weber, Wilfried
    Urban, Gerald A.
    Dincer, Can
    [J]. BIOSENSORS & BIOELECTRONICS, 2021, 177
  • [9] CRISPR/Cas13a-Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification-Free miRNA Diagnostics
    Bruch, Richard
    Baaske, Julia
    Chatelle, Claire
    Meirich, Mailin
    Madlener, Sibylle
    Weber, Wilfried
    Dincer, Can
    Urban, Gerald Anton
    [J]. ADVANCED MATERIALS, 2019, 31 (51)
  • [10] The organization of the human cerebellum estimated by intrinsic functional connectivity
    Buckner, Randy L.
    Krienen, Fenna M.
    Castellanos, Angela
    Diaz, Julio C.
    Yeo, B. T. Thomas
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 2011, 106 (05) : 2322 - 2345
123456