Inducible colistin resistance via a disrupted plasmid-borne mcr-1 gene in a 2008 Vietnamese Shigella sonnei isolate

被引：73

作者：

Duy Pham Thanh ^{[1
]}

Ha Thanh Tuyen ^{[1
]}

To Nguyen Thi Nguyen ^{[1
]}

Hao Chung The ^{[1
]}

Wick, Ryan R. ^{[2
,3
]}

Thwaites, Guy E. ^{[3
,4
]}

Baker, Stephen ^{[3
,4
,5
]}

Holt, Kathryn E. ^{[2
,3
]}

机构：

[1] Univ Oxford, Clin Res Unit, Wellcome Trust Major Overseas Programme, Hosp Trop Dis, Ho Chi Minh City, Vietnam

[2] Univ Melbourne, Ctr Syst Genom, Melbourne, Vic, Australia

[3] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Melbourne, Vic, Australia

[4] Univ Oxford, Ctr Trop Med & Global Hlth, Oxford, England

[5] London Sch Hyg & Trop Med, London, England

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2016年 / 71卷 / 08期

基金：

澳大利亚国家健康与医学研究理事会; 英国惠康基金;

关键词：

ESCHERICHIA-COLI;

D O I：

10.1093/jac/dkw173

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The objective of this study was to assess the presence of mcr-1 in Shigella sonnei isolated in Vietnam. WGS data were analysed for the presence of the mcr-1 gene sequence. The association of mcr-1 with a plasmid was assessed by PCR and by conjugation. Through genome sequencing we identified a plasmid-associated inactive form of mcr-1 in a 2008 Vietnamese isolate of Shigella sonnei. The plasmid was conjugated into Escherichia coli and mcr-1 was activated upon exposure to colistin, resulting in highly colistin-resistant transconjugants. This is the first description of the mcr-1 gene in Shigella, which is atypical given that colistin is not ordinarily used to treat diarrhoea. Our data suggest the mcr-1 gene has been circulating in human-restricted pathogens for some time but likely carries a selective fitness cost.

引用

页码：2314 / 2317

页数：4

共 16 条

[1]

[Anonymous], 2011, 24 INFORMATIONAL SUP

[2]

Arcilla MS, 2016, LANCET INFECT DIS, V16, P147, DOI 10.1016/S1473-3099(15)00541-1

[3] SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing [J].

Bankevich, Anton ;

Nurk, Sergey ;

Antipov, Dmitry ;

Gurevich, Alexey A. ;

Dvorkin, Mikhail ;

Kulikov, Alexander S. ;

Lesin, Valery M. ;

Nikolenko, Sergey I. ;

Son Pham ;

Prjibelski, Andrey D. ;

Pyshkin, Alexey V. ;

Sirotkin, Alexander V. ;

Vyahhi, Nikolay ;

Tesler, Glenn ;

Alekseyev, Max A. ;

Pevzner, Pavel A. .

JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) :455-477

[4] ACT: the Artemis comparison tool [J].

Carver, TJ ;

Rutherford, KM ;

Berriman, M ;

Rajandream, MA ;

Barrell, BG ;

Parkhill, J .

BIOINFORMATICS, 2005, 21 (16) :3422-3423

[5] Co-occurrence of extended spectrum β lactamase and MCR-1 encoding genes on plasmids [J].

Haenni, Marisa ;

Poirel, Laurent ;

Kieffer, Nicolas ;

Chatre, Pierre ;

Saras, Estelle ;

Metayer, Veronique ;

Dumoulin, Romain ;

Nordmann, Patrice ;

Madec, Jean-Yves .

LANCET INFECTIOUS DISEASES, 2016, 16 (03) :281-282

[6] Detection of mcr-1 encoding plasmid-mediated colistin-resistant Escherichia coli isolates from human bloodstream infection and imported chicken meat, Denmark 2015 [J].

Hasman, H. ;

Hammerum, A. M. ;

Hansen, F. ;

Hendriksen, R. S. ;

Olesen, B. ;

Agerso, Y. ;

Zankari, E. ;

Leekitcharoenphon, P. ;

Stegger, M. ;

Kaas, R. S. ;

Cavaco, L. M. ;

Hansen, D. S. ;

Aarestrup, F. M. ;

Skov, R. L. .

EUROSURVEILLANCE, 2015, 20 (49) :2-6

[7] Tracking the establishment of local endemic populations of an emergent enteric pathogen [J].

Holt, Kathryn E. ;

Tran Vu Thieu Nga ;

Duy Pham Thanh ;

Ha Vinh ;

Kim, Dong Wook ;

My Phan Vu Tra ;

Campbell, James I. ;

Nguyen Van Minh Hoang ;

Nguyen Thanh Vinh ;

Pham Van Minh ;

Cao Thu Thuy ;

Tran Thi Thu Nga ;

Thompson, Corinne ;

Tran Thi Ngoc Dung ;

Nguyen Thi Khanh Nhu ;

Phat Voong Vinh ;

Pham Thi Ngoc Tuyet ;

Hoang Le Phuc ;

Nguyen Thi Nam Lien ;

Bui Duc Phu ;

Nguyen Thi Thuy Ai ;

Nguyen Manh Tien ;

Nguyen Dong ;

Parry, Christopher M. ;

Tran Tinh Hien ;

Farrar, Jeremy J. ;

Parkhill, Julian ;

Dougan, Gordon ;

Thomson, Nicholas R. ;

Baker, Stephen .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (43) :17522-17527

[8] SRST2: Rapid genomic surveillance for public health and hospital microbiology labs [J].

Inouye, Michael ;

Dashnow, Harriet ;

Raven, Lesley-Ann ;

Schultz, Mark B. ;

Pope, Bernard J. ;

Tomita, Takehiro ;

Zobel, Justin ;

Holt, Kathryn E. .

GENOME MEDICINE, 2014, 6

[9]

Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/NMETH.1923, 10.1038/nmeth.1923]

[10] Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study [J].

Liu, Yi-Yun ;

Wang, Yang ;

Walsh, Timothy R. ;

Yi, Ling-Xian ;

Zhang, Rong ;

Spencer, James ;

Doi, Yohei ;

Tian, Guobao ;

Dong, Baolei ;

Huang, Xianhui ;

Yu, Lin-Feng ;

Gu, Danxia ;

Ren, Hongwei ;

Chen, Xiaojie ;

Lv, Luchao ;

He, Dandan ;

Zhou, Hongwei ;

Liang, Zisen ;

Liu, Jian-Hua ;

Shen, Jianzhong .

LANCET INFECTIOUS DISEASES, 2016, 16 (02) :161-168

← 1 2 →