Cytotoxic and Antioxidant Activities of Imine Analogs of Trans-Resveratrol towards Murine Neuronal N2a Cells

被引：2

作者：

Ksila, Mohamed ^{[1
,2
]}

Vejux, Anne ^{[1
]}

Prost-Camus, Emmanuelle ^{[3
]}

Durand, Philippe ^{[3
]}

Ghzaiel, Imen ^{[1
,4
]}

Nury, Thomas ^{[1
]}

Duprey, Dorian ^{[5
]}

Meziane, Smail ^{[4
]}

Masmoudi-Kouki, Olfa ^{[2
]}

Latruffe, Norbert ^{[1
]}

Ghrairi, Taoufik ^{[2
]}

Prost, Michel ^{[3
]}

Lizard, Gerard ^{[1
]}

Vervandier-Fasseur, Dominique ^{[5
]}

机构：

[1] Univ Bourgogne Franche Comte, Team Biochem Peroxisome Inflammat & Lipid Metab, EA7270, INSERM, F-21000 Dijon, France

[2] Univ Tunis El Manar, Fac Sci, Dept Biol, Lab Neurophysiol Cellular Physiopathol & Valorisa, LR18ES03, Tunis 2092, Tunisia

[3] Lab Spiral, F-21560 Couternon, France

[4] Inst Europeen Antioxydants IEA, 1B,Rue Victor de Lespinats, F-54230 Neuves Maisons, France

[5] Univ Bourgogne Franche Comte, Inst Mol Chem, Team OCS, Univ Burgundy,ICMUB UMR CNRS 6302, F-21000 Dijon, France

来源：

MOLECULES | 2022年 / 27卷 / 15期

关键词：

trans-resveratrol; aza-stilbenes synthesis; antioxidant activity; cytotoxicity; murine neuronal N2a cells; POLYPHENOLS; DERIVATIVES; HYDROXYL; CRYSTAL;

D O I：

10.3390/molecules27154713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trans-resveratrol is a natural polyphenol showing numerous biological properties, especially anti-tumoral and antioxidant activity. Among numerous resveratrol derivatives, aza-stilbenes, which bear an imine bound, show interesting biological activities. In the present study, we synthesized a series of imine analogs of trans-resveratrol (seven aza-stilbenes) following an easy and low-cost procedure of green chemistry. The toxicity of synthesized aza-stilbenes, which is currently unknown, was evaluated on murine neuronal N2a cells, comparatively to trans-resveratrol, by considering: cell density evaluated by staining with sulforhodamine 101; esterase activity, which is a criteria of cell viability, by staining with fluorescein diacetate; and transmembrane mitochondrial potential, which is known to decrease during cell death, by staining with DiOC(6)(3) using flow cytometry. In addition, the antioxidant activity was quantified with the KRL (Kit Radicaux Libres) assay, the DPPH (2,2 '-diphenyl-1-picrylhydrazyl radical) assay and the FRAP (ferric reducing antioxidant power) assay. The PAOT (Pouvoir Antioxidant Total) score was also used. The aza-stilbenes provide different cytotoxic and antioxidant activities, which are either higher or lower than those of trans-resveratrol. Based on their cytotoxic and antioxidant characteristics, all synthesized aza-stilbenes are distinguished from trans-resveratrol.

引用

页数：16