Using ABCG2-molecule-expressing side population cells to identify cancer stem-like cells in a human ovarian cell line

被引：45

作者：

Dou, Jun ^{[1
]}

Jiang, Cuilian ^{[2
]}

Wang, Jing ^{[3
]}

Zhang, Xian ^{[3
]}

Zhao, Fengshu ^{[1
]}

Hu, Weihua ^{[1
]}

He, Xiangfeng ^{[1
]}

Li, Xiaoli ^{[1
]}

Zou, Dandan ^{[1
]}

Gu, Ning ^{[4
]}

机构：

[1] Southeast Univ, Sch Med, Dept Pathogen Biol & Immunol, Nanjing 210009, Peoples R China

[2] Maternal & Child Hlth, Nanjing 210008, Peoples R China

[3] Southeast Univ, Zhongda Hosp, Sch Med, Nanjing 210009, Peoples R China

[4] Southeast Univ, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2011年 / 35卷 / 03期

基金：

中国国家自然科学基金;

关键词：

ABCG2; molecule; cancer stem cell; cancer stem-like cell; ovarian cancer; side population cell; INITIATING CELLS; IDENTIFICATION; LUNG; PROLIFERATION; SURVIVAL; CD133;

D O I：

10.1042/CBI20100347

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

CSCs (cancer stem cells) are a small subset of cells within a tumour that possesses the characteristics of stem cells and are considered to be responsible for resistance to chemoradiation. Identification of CSCs through stem cell characteristics might have relevant clinical implications. In this study, SP (side population) cells were sorted from a human ovarian cancer cell line by FACS to determine whether cancer stem cell-like SP cells were present. A very small fraction of SP cells (2.6%) was detected in A2780 cells. SP cells possessed the following characteristics: highly proliferative activity, marked ability for self-renewal in soft agar and culture medium, high expression of ABCG2, drug resistance to vinblastine in vitro, and strong tumourigenic potential in Balb/c nude mice. It is concluded that there exists in the A2780 cell line a small number of SP cells with high expression of ABCG2. The cells have the characteristics of cancer stem-like cells, and identification and cloning of such human SP cells can help in improving therapeutic approaches to ovarian cancer in patients.

引用

页码：227 / 234

页数：8

共 33 条

[11] Ovarian cancer stem-like side-population cells are tumourigenic and chemoresistant [J].

Hu, L. ;

McArthur, C. ;

Jaffe, R. B. .

BRITISH JOURNAL OF CANCER, 2010, 102 (08) :1276-1283

[12] Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro [J].

Ignatova, TN ;

Kukekov, VG ;

Laywell, ED ;

Suslov, ON ;

Vrionis, FD ;

Steindler, DA .

GLIA, 2002, 39 (03) :193-206

[13]

JUN D, 2009, CELL BIOL INT, V33, P807

[14]

JUN D, 2009, CELL MOL BIOL NOIS S, V55

[15]

JUN D, 2006, J GASTROEN HEPATOL, V21, P1794

[16]

JUN D, 2007, CELL MOL IMMUNOL, V4, P528

[17] Identification of bronchioalveolar stem cells in normal lung and lung cancer [J].

Kim, CFB ;

Jackson, EL ;

Woolfenden, AE ;

Lawrence, S ;

Babar, I ;

Vogel, S ;

Crowley, D ;

Bronson, RT ;

Jacks, T .

CELL, 2005, 121 (06) :823-835

[18]

Kim M, 2002, CLIN CANCER RES, V8, P22

[19] CD133-Expressing Stem Cells Associated with Ovarian Metastases Establish an Endothelial Hierarchy and Contribute to Tumor Vasculature [J].

Kusumbe, Anjali P. ;

Mali, Avinash M. ;

Bapat, Sharmila A. .

STEM CELLS, 2009, 27 (03) :498-508

[20] The biology of cancer stem cells [J].

Lobo, Neethan A. ;

Shimono, Yohei ;

Qian, Dalong ;

Clarke, Michael F. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2007, 23 :675-699

← 1 2 3 4 →