MicroRNA-449a acts as a tumor suppressor in human bladder cancer through the regulation of pocket proteins

被引：93

作者：

Chen, Hong ^{[1
]}

Lin, Yi-Wei ^{[1
]}

Mao, Ye-Qing ^{[1
]}

Wu, Jian ^{[1
]}

Liu, Yun-Fu ^{[1
]}

Zheng, Xiang-Yi ^{[1
]}

Xie, Li-Ping ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Urol, Hangzhou 310003, Zhejiang, Peoples R China

来源：

CANCER LETTERS | 2012年 / 320卷 / 01期

基金：

中国国家自然科学基金;

关键词：

miR-449a; Bladder cancer; Cell cycle; Pocket protein; Targeted therapy; CELL-PROLIFERATION; PHOSPHORYLATION; TARGETS; EXPRESSION; MIR-449A; P130; TRANSITION; ARREST; FAMILY; E2F1;

D O I：

10.1016/j.canlet.2012.01.027

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Frequent downregulation of microRNA-449a (miR-449a) was detected in 14 human bladder cancer tissues. The restoration of miR-449a inhibited cell growth and induced G1-phase arrest in T24 and 5637 human bladder cancer cells. CDK6 and CDC25a were downregulated after miR-449a treatment, resulting in the functional accumulation of the pocket proteins Rb and p130. The growth of 124 tumor xenografts was suppressed by exogenous miR-449a, and the nuclear proliferation antigen Ki-67 was downregulated in miR-449a-treated tumors. These results suggest a tumor-suppressive role for miR-449a in human bladder cancer. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：40 / 47

页数：8

共 39 条

[21] Control of vertebrate multiciliogenesis by miR-449 through direct repression of the Delta/Notch pathway [J].

Marcet, Brice ;

Chevalier, Benoit ;

Luxardi, Guillaume ;

Coraux, Christelle ;

Zaragosi, Laure-Emmanuelle ;

Cibois, Marie ;

Robbe-Sermesant, Karine ;

Jolly, Thomas ;

Cardinaud, Bruno ;

Moreilhon, Chimene ;

Giovannini-Chami, Lisa ;

Nawrocki-Raby, Beatrice ;

Birembaut, Philippe ;

Waldmann, Rainer ;

Kodjabachian, Laurent ;

Barbry, Pascal .

NATURE CELL BIOLOGY, 2011, 13 (06) :693-U157

[22] Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells [J].

Marson, Alexander ;

Levine, Stuart S. ;

Cole, Megan F. ;

Frampton, Garrett M. ;

Brambrink, Tobias ;

Johnstone, Sarah ;

Guenther, Matthew G. ;

Johnston, Wendy K. ;

Wernig, Marius ;

Newman, Jamie ;

Calabrese, J. Mauro ;

Dennis, Lucas M. ;

Volkert, Thomas L. ;

Gupta, Sumeet ;

Love, Jennifer ;

Hannett, Nancy ;

Sharp, Phillip A. ;

Bartel, David P. ;

Jaenisch, Rudolf ;

Young, Richard A. .

CELL, 2008, 134 (03) :521-533

[23]

Mayol X, 1996, ONCOGENE, V13, P237

[24]

MAYOL X, 1995, ONCOGENE, V11, P801

[25] A microRNA expression ratio defining the invasive phenotype in bladder tumors [J].

Neely, Lori A. ;

Rieger-Christ, Kimberly M. ;

Silva Neto, Brasil ;

Eroshkin, Alexey ;

Garver, Joanne ;

Patel, Sonal ;

Nu Ai Phung ;

McLaughlin, Stephen ;

Libertino, John A. ;

Whitney, Duncan ;

Summerhayes, Ian C. .

UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS, 2010, 28 (01) :39-48

[26] miR-449a targets HDAC-1 and induces growth arrest in prostate cancer [J].

Noonan, E. J. ;

Place, R. F. ;

Pookot, D. ;

Basak, S. ;

Whitson, J. M. ;

Hirata, H. ;

Giardina, C. ;

Dahiya, R. .

ONCOGENE, 2009, 28 (14) :1714-1724

[27] miR-449a causes Rb-dependent cell cycle arrest and senescence in prostate cancer cells [J].

Noonan, Emily J. ;

Place, Robert F. ;

Basak, Shashwati ;

Pookot, Deepa ;

Li, Long-Cheng .

ONCOTARGET, 2010, 1 (05) :349-358

[28] Chromatin structure analyses identify miRNA promoters [J].

Ozsolak, Fatih ;

Poling, Laura L. ;

Wang, Zhengxin ;

Liu, Hui ;

Liu, X. Shirley ;

Roeder, Robert G. ;

Zhang, Xinmin ;

Song, Jun S. ;

Fisher, David E. .

GENES & DEVELOPMENT, 2008, 22 (22) :3172-3183

[29] The present and future burden of urinary bladder cancer in the world [J].

Ploeg, Martine ;

Aben, Katja K. H. ;

Kiemeney, Lambertus A. .

WORLD JOURNAL OF UROLOGY, 2009, 27 (03) :289-293

[30] HUMAN BLADDER-CARCINOMA CELL-LINES AS INDICATORS OF ONCOGENIC CHANGE RELEVANT TO UROTHELIAL NEOPLASTIC PROGRESSION [J].

RIEGER, KM ;

LITTLE, AR ;

SWART, JM ;

KASTRINAKIS, WV ;

FITZGERALD, JM ;

HESS, DT ;

LIBERTINO, JA ;

SUMMERHAYES, IC .

BRITISH JOURNAL OF CANCER, 1995, 72 (03) :683-690

← 1 2 3 4 →