Biomimetic interactions of proteins with functionalized cadmium sulfide quantum dots

被引：38

作者：

Huang, Deping ^{[1
]}

Geng, Fei ^{[1
]}

Liu, Yonghui ^{[1
]}

Wang, Xiaoqing ^{[1
]}

Jiao, Jingjing ^{[1
]}

Yu, Li ^{[1
]}

机构：

[1] Shandong Univ, Key Lab Colloid & Interface Chem, Minist Educ, Jinan 250100, Peoples R China

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2011年 / 392卷 / 01期

关键词：

Interaction; Bovine serum albumin; Lysozyme; Capping agent; CdS QDs; Circular dichroism; Fluorescence; BOVINE SERUM-ALBUMIN; CDS NANOCRYSTALS; HUMAN HEMOGLOBIN; ENERGY-TRANSFER; SOLAR-CELLS; BINDING; CDTE; OLIGONUCLEOTIDES; NANOPARTICLES; PEPTIDES;

D O I：

10.1016/j.colsurfa.2011.09.054

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Interactions between various modified semiconductor nanocrystal, cadmium sulfide quantum dots (CdS QDs) and bovine serum albumin (BSA) and lysozyme (LZY) were investigated. CdS QDs capped with mercaptoethanol (MPA), L-cysteine (Lcys) and glutathione (GSH) were synthesized in aqueous solution and characterized by UV-vis and fluorescence spectrum. Circular dichroism (CD) and fluorescence spectrum were used to detect the interactions between as-prepared CdS QDs and protein molecules. The interaction parameters, including binding constant (K-b), binding site number (n) and quench constant (K-q), were determined by fluorescence spectrum. The changes of secondary structures of the proteins were detected by CD. The results imply that CdS QDs modified by different agents have dramatically different binding strength with protein molecules. The results obtained here analyze the biosafety of CdS QDs in terms of the biological behavior of biomolecules and could serve as basis for the application of CdS QDs to bioscience. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：191 / 197

页数：7

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