Analysis of urinary caffeine metabolites by HPLC-DAD: the use of metabolic ratios to assess CYP1A2 enzyme activity

被引：30

作者：

Caubet, MS ^{[1
]}

Elbast, W ^{[1
]}

Dubuc, MC ^{[1
]}

Brazier, JL ^{[1
]}

机构：

[1] Hop St Justine, Ctr Rech, Fac Pharm, Chaire Med Grossesse & Allaitement, Montreal, PQ H3C 3J7, Canada

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2002年 / 27卷 / 1-2期

关键词：

caffeine; caffeine metabolites ratios; phenotyping; CYP1A2;

D O I：

10.1016/S0731-7085(01)00546-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An improved extraction procedure and a new RP-HPLC method were developed for selective and rapid analysis of caffeine and 14 of its metabolites in urine. Analytes were isolated by solid-phase extraction and separated on an Eclipse XDB-C18 column. Recoveries ranged between 83 and 99%. Precision, linearity and accuracy of the chromatographic method were found to be within required limits. Using this procedure, caffeine metabolic ratios were determined in 20 subjects with characteristic CYP1A2 activities, relatively to smoking habit and contraceptives intake. The method might be useful to point out induction and inhibition of CYP1A2 activity. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：261 / 270

页数：10

共 19 条

[1] COMPARISON OF CAFFEINE METABOLISM BY SLICES, MICROSOMES AND HEPATOCYTE CULTURES FROM ADULT HUMAN-LIVER [J].