An electrochemical multienzymatic biosensor for determination of cholesterol

被引：69

作者：

Bongiovanni, C

Ferri, T

Poscia, A

Varalli, M

Santucci, R

Desideri, A

机构：

[1] Univ Roma Tor Vergata, Dipartimento Biol, I-00133 Rome, Italy

[2] Univ Roma Tor Vergata, INFM, I-00133 Rome, Italy

[3] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[4] Menarini Ind Farmaceut Riunite SRL, Florence, Italy

[5] Univ Roma Tor Vergata, Dipartimento Med Sperimentale & Sci Biochim, I-00133 Rome, Italy

来源：

BIOELECTROCHEMISTRY | 2001年 / 54卷 / 01期

关键词：

cholesterol; biosensor; cyclic voltammetry; flow injection analysis; horseradish peroxidase;

D O I：

10.1016/S0302-4598(01)00105-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film. on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：17 / 22

页数：6

共 22 条

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ADEIRAN SA, 1989, EUR J BIOCHEM, V186, P571

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