The Antioxidant and Antitumor Efficiency of Litophyton sp. Extract in DMH-Induced Colon Cancer in Male Rats

被引:8
作者
Ashry, Mahmoud [1 ]
Askar, Hussam [1 ]
Alian, Abdallah [1 ]
Zidan, Sabry A. H. [2 ]
El-Sahra, Doaa G. [3 ]
Abdel-Wahhab, Khaled G. [4 ]
Lamlom, Sobhi F. [5 ]
Abdelsalam, Nader R. [6 ]
Abd El-Hack, Mohamed E. [7 ]
Gomaa, Heba F. [8 ]
机构
[1] Al Azhar Univ, Fac Sci, Zool Dept, Assiut 71524, Egypt
[2] Al Azhar Univ, Fac Pharm, Dept Pharmacognosy, Assiut 71524, Egypt
[3] Modern Univ Technol & Informat, Fac Nursing, Med Surg Nursing Dept, Cairo 12055, Egypt
[4] Natl Res Ctr, Med Physiol Dept, Giza 12622, Egypt
[5] Alexandria Univ, Fac Agr Saba Basha, Plant Prod Dept, Alexandria 21531, Egypt
[6] Alexandria Univ, Fac Agr Saba Basha, Agr Bot Dept, Alexandria 21531, Egypt
[7] Zagazig Univ, Fac Agr, Dept Poultry, Zagazig 44511, Egypt
[8] Ain Shams Univ, Fac Sci, Dept Zool, Cairo 11566, Egypt
来源
LIFE-BASEL | 2022年 / 12卷 / 10期
关键词
colon cancer; DMH; antitumor; Litophyton sp; soft coral; oxidative stress; TUMOR-NECROSIS-FACTOR; LIPID-PEROXIDATION; NITRIC-OXIDE; APOPTOSIS; BIOLOGY;
D O I
10.3390/life12101470
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
One of the most common tumors to cause death worldwide is colon cancer. This study aims to investigate the antitumor potency of Litophyton sp. methanolic extract (LME) against DMH-induced colon cancer in adult male rats. Group (1) normal rats served as the control, group (2) normal rats were ip-injected with LME at a dose of 100 mu g/kg/day, group (3) DMH-induced colon cancer animals, and group (4) colon cancer-modeled animals were treated with LME (100 mu g/kg/day) for six weeks. The results revealed that injection of LME markedly regenerated the colon cancer pathophysiological disorders; this was monitored from the significant reduction in the values of serum biomarkers (CEA, CA19.9, AFP), cytokines (TNF-alpha and IL1 beta), and biochemical measurements (ALAT, ASAT, urea, creatinine, cholesterol, and triglycerides) matched significant increase of apoptotic biomarkers (CD4+); similarly, colon DNA fragmentation, MDA, and NO levels were down-regulated. In contrast, a remarkable upregulation in colon SOD, GPx, GSH, and CAT levels was noted. Moreover, the colon histopathological architecture showed obvious regenerations. Chromatography of LME resulted in the purification of two polyhydroxylated steroids (1 and 2) with potential cytotoxic activities. LME performed therapeutic potential colon tumorigenesis; therefore, LME may have a promising chemo-preventive feature against colon cancer, probably via enhancement of the apoptosis pathway, improvement of the immune response, reduction of inflammation, or/and restoration of the impaired oxidative stress.
引用
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页数:24
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