Mapping molecular complexes with super-resolution microscopy and single-particle analysis

被引：4
作者：
Mendes, Afonso [1 ]
Heil, Hannah S. [1 ]
Coelho, Simao [1 ]
Leterrier, Christophe [2 ]
Henriques, Ricardo [1 ,3 ]
机构：
[1] Inst Gulbenkian Ciencias, Oeiras, Portugal
[2] Aix Marseille Univ, CNRS, INP, UMR7051,NeuroCyto, Marseille, France
[3] UCL, MRC Lab Mol Cell Biol, London, England
来源：
OPEN BIOLOGY | 2022年 / 12卷 / 07期
基金：
欧洲研究理事会; 英国惠康基金; 英国医学研究理事会; 欧盟地平线“2020”;
关键词：
structural biology; super-resolution microscopy; single-particle analysis; NUCLEAR-PORE COMPLEX; OPTICAL RECONSTRUCTION MICROSCOPY; HERPES-SIMPLEX-VIRUS; FLUORESCENCE MICROSCOPY; 3-DIMENSIONAL STRUCTURE; PRIMARY CILIOGENESIS; BASAL BODIES; ARCHITECTURE; REVEALS; CENTROSOME;
D O I：
10.1098/rsob.220079
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM-SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.
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页数：12
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