Mapping molecular complexes with super-resolution microscopy and single-particle analysis

被引：4
作者：
Mendes, Afonso [1 ]
Heil, Hannah S. [1 ]
Coelho, Simao [1 ]
Leterrier, Christophe [2 ]
Henriques, Ricardo [1 ,3 ]
机构：
[1] Inst Gulbenkian Ciencias, Oeiras, Portugal
[2] Aix Marseille Univ, CNRS, INP, UMR7051,NeuroCyto, Marseille, France
[3] UCL, MRC Lab Mol Cell Biol, London, England
来源：
OPEN BIOLOGY | 2022年 / 12卷 / 07期
基金：
欧洲研究理事会; 英国惠康基金; 英国医学研究理事会; 欧盟地平线“2020”;
关键词：
structural biology; super-resolution microscopy; single-particle analysis; NUCLEAR-PORE COMPLEX; OPTICAL RECONSTRUCTION MICROSCOPY; HERPES-SIMPLEX-VIRUS; FLUORESCENCE MICROSCOPY; 3-DIMENSIONAL STRUCTURE; PRIMARY CILIOGENESIS; BASAL BODIES; ARCHITECTURE; REVEALS; CENTROSOME;
D O I：
10.1098/rsob.220079
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM-SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.
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页数：12
相关论文
共 113 条
  • [1] Abbe E., 1873, ARCH MIKROSK ANAT, V9, P413, DOI [10.1007/BF02956173, DOI 10.1007/BF02956173]
  • [2] The molecular architecture of the nuclear pore complex
    Alber, Frank
    Dokudovskaya, Svetlana
    Veenhoff, Liesbeth M.
    Zhang, Wenzhu
    Kipper, Julia
    Devos, Damien
    Suprapto, Adisetyantari
    Karni-Schmidt, Orit
    Williams, Rosemary
    Chait, Brian T.
    Sali, Andrej
    Rout, Michael P.
    [J]. NATURE, 2007, 450 (7170) : 695 - 701
  • [3] 3-DIMENSIONAL STRUCTURE OF BASAL BODY FROM RHESUS-MONKEY OVIDUCT
    ANDERSON, RG
    [J]. JOURNAL OF CELL BIOLOGY, 1972, 54 (02) : 246 - &
  • [4] nanoTRON: a Picasso module for MLP-based classification of super-resolution data
    Auer, Alexander
    Strauss, Maximilian T.
    Strauss, Sebastian
    Jungmann, Ralf
    [J]. BIOINFORMATICS, 2020, 36 (11) : 3620 - 3622
  • [5] Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes
    Balzarotti, Francisco
    Eilers, Yvan
    Gwosch, Klaus C.
    Gynna, Arvid H.
    Westphal, Volker
    Stefani, Fernando D.
    Elf, Johan
    Hell, Stefan W.
    [J]. SCIENCE, 2017, 355 (6325) : 606 - 612
  • [6] Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging
    Bauer, Manuel
    Cubizolles, Fabien
    Schmidt, Alexander
    Nigg, Erich A.
    [J]. EMBO JOURNAL, 2016, 35 (19) : 2152 - 2166
  • [7] Local and global analysis of endocytic patch dynamics in fission yeast using a new "temporal superresolution" realignment method
    Berro, Julien
    Pollard, Thomas D.
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2014, 25 (22) : 3501 - 3514
  • [8] Imaging intracellular fluorescent proteins at nanometer resolution
    Betzig, Eric
    Patterson, George H.
    Sougrat, Rachid
    Lindwasser, O. Wolf
    Olenych, Scott
    Bonifacino, Juan S.
    Davidson, Michael W.
    Lippincott-Schwartz, Jennifer
    Hess, Harald F.
    [J]. SCIENCE, 2006, 313 (5793) : 1642 - 1645
  • [9] 3D Structure From 2D Microscopy Images Using Deep Learning
    Blundell, Benjamin
    Sieben, Christian
    Manley, Suliana
    Rosten, Ed
    Ch'ng, Queelim
    Cox, Susan
    [J]. FRONTIERS IN BIOINFORMATICS, 2021, 1
  • [10] The Centrosome in Cells and Organisms
    Bornens, Michel
    [J]. SCIENCE, 2012, 335 (6067) : 422 - 426
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